The unique robotic pick and place arm places one half of the buckets in the centrifuge in order for the system to begin its parallel processing. Sensors are linked to alarms which will alert the user if the centrifuge is out of balance or misloaded. Each bucket has a unique ID on the corresponding loading tray and the corresponding run data is logged which may then be downloaded from the system for audit purposes.
Alternate buckets are swapped out, allowing the second half of the harvest to be spun or processed. This parallel processing feature decreases overall processing time for the harvest, providing more consistent exposure times.
During each step in the harvest, the user can see which process is ongoing by referencing the easy to use LCD screen; it shows which buckets are being centrifuged as well as which step in processing the alternate buckets are in.
Between each aspiration step, the dual probe aspiration head swivels to the wash station which washes with the correct reagent for the respective harvest step in order to clean the probes between aspirations.
Each set of probes has dual tips with automatic blockage sensors and an automatic blockage clearing routine, thereby minimising the effects of any blockage encountered during any stages of the harvest.
The centrifuge lid slides shut with a set of buckets ready for processing. Centrifugation begins under the liquid processing stage. The prefix cycle displayed shows all three towers at work. In the first bucket, the aspiration tower aspirates the hypotonic supernatant, in the subsequent bucket the pellet is resuspended with hypotonic at the second tower and fixative is being dispensed from the third tower into the final bucket.
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