| Streptavidin is a homotetrameric protein with a molecular weight of 55 kDa. It is found in the culture broth of Streptomyces avidinii. Like avidin, one mole of streptavidin can bind 4 moles of biotin with an affinity virtually unmatched in nature. Streptavidin lacks the carbohydrate side chains present in avidin and has an isoelectric point close to neutrality. For this reason, it undergoes less nonspecific binding than avidin. Streptavidin has been extensively applied in various biological applications, such as ELISA, IHC, TRFIA, quantitative PCR, isolation of single-stranded nucleotides, purification of biomolecules, and monoclonal antibody production. |
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