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产品概述

Screen Quest 荧光ELISA cAMP分析试剂盒提供了一种优化的分析方法，用于检测G蛋白偶联受体  ， 系统中腺苷酸环化酶的。该测定基于HRP标记的cAMP与未标记的cAMP之间固定数目的抗体结合位点的竞争。通过未标记的游离cAMP将HRP-cAMP从HRP-cAMP /抗cAMP抗体复合物中置换出来。在不存在cAMP的情况下，HRP-cAMP偶联物与抗cAMP抗体结合。然而，测试样品中未标记的游离cAMP与HRP-cAMP抗体偶联物竞争抗cAMP抗体，因此抑制了HRP-cAMP与抗cAMP抗体的结合。我们的Screen Quest 荧光cAMP分析试剂盒提供了检测腺苷酸环化酶活性的灵敏方法。与其他商用ELISA cAMP分析试剂盒相比，它繁琐的乙酰化步骤。该试剂盒使用Amplite Red作为荧光HRP底物来定量HRP活性。形成的荧光产物与HRP-cAMP偶联物的活性成比例。百萤生物是AAT Bioquest的代理商，为您提供优质的Screen Quest cAMP检测试剂盒。

Screen Quest? Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. The assay is based on the competition for a fixed number of antibody binding sites between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest? Fluorometric cAMP Assay Kit provides a sensitive method for detecting adenylate cyclase activity. Compared to other commercial ELISA cAMP assay kits, this cAMP assay kit only requires a single wash step to remove unbound material prior to the development step. It also eliminates the tedious acetylation step. The kit uses Amplite? Red as a fluorogenic HRP substrate to quantify the HRP activity. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate.