Screen Quest 免洗cAMP检测试剂盒提供了一种便捷的分析方法，用于检测G蛋白偶联受体，系统中腺苷酸环化酶的活化。与其他商用ELISA cAMP分析试剂盒相比，这种均质的cAMP分析试剂盒不需要洗涤步骤或乙酰化步骤。该测定基于荧光cAMP示踪剂和未标记的游离cAMP之间固定数目的抗cAMP抗体结合位点的竞争。游离cAMP从HRP-cAMP /抗cAMP抗体复合物中取代了荧光cAMP示踪剂。抗cAMP抗体用我们的TR Fluor Eu标记，而cAMP示踪剂包含我们的trFluor 650。在没有cAMP的情况下，trFluor 650-cAMP缀合物与TR Fluor Eu标记的抗cAMP抗体结合。但是，测试样品中未标记的游离cAMP竞争TR Fluor Eu标记的抗cAMP抗体偶联物，因此抑制了trFluor 650-cAMP与抗cAMP抗体的结合。 trFluor 650标记的cAMP示踪剂具有纳秒级的荧光寿命，而TR Fluor Eu标记的抗cAMP抗体结合的荧光cAMP示踪剂由于TR-FRET而具有更长的荧光寿命值。 FRET的大小与样品中cAMP的浓度成正比。该测定可以使用96孔或384孔微孔板进行。百萤生物是AAT Bioquest的代理商，为您提供优质的Screen Quest 免洗cAMP检测试剂盒。
As our kit 36376, Screen Quest? No Wash FRET cAMP Assay Kit provides another convenient assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. Compared to other commercial ELISA cAMP assay kits, this homogenous cAMP assay kit does not require a wash step or the acetylation step. The assay is based on the competition for a fixed number of anti-cAMP antibody binding sites between the fluorescent cAMP tracer and non-labeled free cAMP. Free cAMP displaces the fluorescent cAMP tracer from the HRP-cAMP/anti-cAMP antibody complex. The anti-cAMP antibody is labeled with our TR Fluor? Tb while the cAMP tracer contains our bFluor? 650. In the absence of cAMP, bFluor? 650-cAMP conjugate is bound to TR Fluor? Tb-labeled anti-cAMP antibody exclusively to have a strong FRET. However, the unlabeled free cAMP in the test sample competes for the TR Fluor? Tb-labeled anti-cAMP antibody conjugate, therefore inhibits the binding of bFluor? 650-cAMP to anti-cAMP antibody. The bFluor? 650 labeled cAMP tracer only has fluorescence lifetime of nanosecond while TR Fluor? Tb-labeled anti-cAMP antibody-bound fluorescent cAMP tracer has much longer fluorescence lifetime value due to the TR-FRET. The magnitude of FRET is proportional to the concentration of cAMP in a sample. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
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