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产品介绍

Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis). It is a major source for methyl groups via its metabolite, trimethylglycine that participates in the S-adenosylmethionine synthesis pathways. Choline deficiency may cause liver disease, atherosclerosis and possibly neurological disorders. Despite its importance in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine, the role of choline in mental illness has been little studied. This Amplite? Choline Quantitation Kit provides one of the most sensitive methods for quantifying choline. The kit uses Amplite? Red to quantify the concentration of choline, which is related to the production of hydrogen peroxide in the choline oxidase-mediated enzyme coupling reactions. The amount of choline is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. In the presence of peroxidase, the fluorescence intensity of Amplite? Red is proportional to the formation of hydrogen peroxide that is converted to the concentration of choline. The assay can be readily read with a fluorescence microplate reader at Ex/Em = ~540/590 nm. Alternatively the assay can also be read at ~570 nm with an absorption microplate reader.

产品概述

胆碱及其代谢产物在细胞膜的结构完整性和信号传导以及胆碱能神经传递（乙酰胆碱合成）中起着重要作用。它是通过其代谢产物三甲基甘氨酸参与S-腺苷甲硫氨酸合成途径而获得甲基的主要来源。胆碱缺乏症可能导致肝脏疾病，动脉粥样硬化以及可能的神经系统疾病。尽管胆碱在中枢神经系统中作为乙酰胆碱和膜磷脂酰胆碱的前体具有重要作用，但胆碱在精神疾病中的作用尚未得到研究。该Amplite 胆碱定量试剂盒提供了灵敏的胆碱定量方法之一。该试剂盒使用Amplite Red量化胆碱的浓度，这与胆碱氧化酶介导的酶偶联反应中过氧化氢的产生有关。胆碱的量与在酶偶联反应循环中形成的过氧化氢的浓度成比例。在存在过氧化物酶的情况下，Amplite Red的荧光强度与过氧化氢的形成成比例，该过氧化氢转化为胆碱的浓度。该测定可以容易地用荧光酶标仪读取，或者也可以使用光吸收酶标仪在?570 nm处读取测定值。百萤生物是AAT Bioquest的代理商，为您提供优质的Amplite 胆碱定量试剂盒。