产品介绍
此离心柱式试剂盒可从琼脂糖凝胶或酶反应液中回收DNA。只要5min即可回收所有类型琼脂糖凝胶或缓冲液系统的DNA。无需为获得更高回收率而特别适用低熔点琼脂糖。本试剂盒会去除掉反应体系中的所有盐分、缓冲液、酶、溴化乙锭、标记物和dNTPs。最终DNA可直接用于下游实验。
UltraClean? GelSpin? DNA Extraction Kit使用了硅胶离心柱滤膜从琼脂糖凝胶中回收DNA。电泳跑完后，切取目标条带，直接放入Spin Filter中。切胶在高离序液盐作用下不可逆溶解。DNA选择性吸附到硅胶滤膜上，冲洗去掉琼脂糖和盐等杂质。最终DNA从离心柱滤膜上洗脱到水或Tris 缓冲液中。
大于0.2g的凝胶片段
把胶放到2ml Tube管中，做步骤2、5、和6。步骤5后取700μl到Spin Filter中。离心10s。再次加载滤液到Spin Filter，离心10s。弃去所有滤液，加载剩余溶解了的凝胶到Spin Filter。再次离心10s。重新加载滤液。离心10s。接着步骤10继续。
文献
Michael S. Robeson, Andrew J. King, Kristen R. Freeman, C. WilliamBirky, Jr, Andrew P. Martin, and Steven K. Schmidt. Soil rotifer communities are extremely diverse globally but spatiallyautocorrelated locally. PNAS, Mar 2011; 108: 4406 - 4410.
Liis Uusküla, Kristiina Rull, Liina Nagirnaja, and Maris Laan. Methylation Allelic Polymorphism (MAP) in Chorionic Gonadotropin β5(CGB5) and Its Association with Pregnancy Success. J. Clin. Endocrinol.Metab., Jan 2011; 96: E199 - E207.
James J. Polashock, Rajeev Arora, Yanhui Peng, Dhananjay Naik, andLisa J. Rowland. Functional Identification of a C-repeat Binding FactorTnaldc.nal.usda.gov/catalog/41923ranscriptional Activator from BlueberryAssociated with Cold Acclimation and Freezing Tolerance J. Amer. Soc. Hort. Sci.,Jan 2010; 135: 40 - 48.
来宝会员