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产品介绍

应用：

TriPure Isolation Reagent can prepare total RNA, genomic DNA, and protein from a single biological sample. After purification:

• The DNA-free total RNA is suitable for northern blotting, in vitro translation, RNase protection assay, cDNA synthesis, or RT-PCR.
• The RNA-free DNA is suitable for PCR, restriction digests, Southern blotting, or cloning.
• The denatured protein is suitable for SDS-PAGE and western blotting.

原理：

During a one-step sample homogenization/lysis procedure, the TriPure Isolation Reagent disrupts cells and denatures endogenous nucleases. Chloroform is added to the sample and the mixture is centrifuged. This separates the sample into three phases: a colorless aqueous (upper) phase, a white interphase and a red organic (lower) phase. The upper phase is placed in a separate tube and RNA is recovered from it by isopropanol precipitation. DNA and protein are sequentially isolated from the remaining white interphase and red organic phase by alcohol precipitation steps.

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