Synonym
IL4, BCGF1, BSF1, MGC79402, Interleukin 4
Source
Recombinant Human IL-4 /Interleukin-4 Protein, HEK293 expressed(rhIL-4), His25-Ser153 (Accession# AAH67514)was expressed in human 293 cells (HEK293) at ACRObiosystems.
Molecular Characterization
rhIL-4 contains no “tag” and has a calculated MW of 14.9 kDa. The predicted N-terminus is His25. DTT-reduced protein migrates as 15 and 19 kDa protein due to glycosylation.
Endotoxin
Less than 1.0 EU per μg of the or rhIL-4 by the LAL method.
Purity
>95% purity as determined by SDS-PAGE of reduced rhIL-4.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM MES, 200 mM NaCl, pH6.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE
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The purity of rhIL-4 was determined by SDS-PAGE of reduced rhIL-4 and staining overnight with Coomassie Blue.
Bioactivity
The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells.
The ED50<0.2ng/ml, corresponding to a specific activity of >5X106 Unit/mg
Background
Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells)[1]. In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated [2], And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine[3]. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen[4].
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References
(1) Callard RE., et al., 1991, Br J Haematol. Jul;78(3):293-9.
(2) Varin A, Gordon S., et al., 2009, Immunobiology. 214(7):630-41.
(3) Finkelman FD, et al., 2004, Immunol Rev. 201:139-55.
(4) Lee YJ, Jameson SC,et al., 2011, Trends Immunol. 32(2):50-6. Epub 2011 Feb 1.
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