产品介绍
Synonym
IGF-1R, IGF1R, CD221, IGFIR, IGFⅠR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, Insulin-like Growth Factor 1 (IGF-1) Receptor
Source
Recombinant Human IGF1R /CD221 Protein (rhIGF1R) Glu31-Asn 932 (Accession # P08069 ) was produced in human 293 cells (HEK293) at ACRObiosystems.
Molecular Characterization
rhIGF1R contains C-terminal polyhistidine tag and has a calculated MW of 104 kDa (single chain), 80 kDa (α subunit) and 23 kDa (β subunit). DTT-reduced protein migrates as 120 kDa, 80 kDa and 35 kDa polypeptide in SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg of the rhIGF1R by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE
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The purity of rhIGF1R was determined by SDS-PAGE of reduced rhIGF1R and staining overnight with Coomassie Blue.
Bioactivity
Measured by its ability to bind human IGF-I in a functional ELISA.
Background
The Insulin-like Growth Factor 1 Receptor （IGF1R）is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.[1] The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.[2-3]
References
(1) Gregory CW, DeGeorges A, Sikes RA (2001). Recent Research Developments in Cancer: 437–462.
(2) Jones JI, Clemmons DR (1995). Endocr. Rev. 16 (1): 3–34.
(3) LeRoith D, et al. (1995). Endocr. Rev. 16 (2): 143–63.
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