Synonym
IGFBP3, IGFBP-3, BP-53, IBP3, IBP-3
Source
Recombinant human IGFBP3 (rhIGFBP3) Gly28-Lys291 (Accession #AAH18962 ) was expressed HEK 293 Cells at ACRObiosystems.
Molecular Characterization
rhIGFBP3 protein contains C-terminal 6XHis tag and has a calculated MW of 31 kDa. DTT-reduced protein migrates as 40-45 kDa polypeptide in SDS-PAGE.
Endotoxin
Less than 1.0 EU per 1 μg of the rhIGFBP3 by the LAL method.
Purity
>95% as determined by SDS-PAGE. All lots are greater than 95% pure.
Formulation
Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE
<img alt="IGFBP3".com/products/IGFBP3-IG3-H5229.jpg" width="130" height="235" />
The purity of rhIGFBP3 was determined by SDS-PAGE of reduced rhIGFBP3 and staining overnight with Coomassie Blue.
Background
Insulin-like growth factor-binding protein 3, also known as IGFBP3, is a protein that, in humans, is encoded by the IGFBP3 gene.[1][2] IGFBP3 forms a ternary complex of about 140 ~150 kDa with IGF1 or IGF2 and a glycoprotein insulin-like growth factor acid-labile subunit (ALS), thus alter the interaction of IGFs with their cell surface receptors. IGFBP3 exerts either proapoptotic or growth stimulatory effects depending upon the cellular context. Studies have shown that IGFBP3 can leads to the induction of apoptosis dependent or independent of the IGF-IGF receptor axis, accordingly acts as a negative regulator of tumorigenesis and progressing in certain carcinomas. The highest expression level is found in the nonparanchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty.
References
(1) Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3
(2) Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619
(3) Spagnoli, A. and R.G. Rosenfeld (1997) Curr. Op. Endocrinology and Diabetes 4:1
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