产品介绍
Synonym
ICAM1, ICAM-1, BB2, BB-2, CD54, CD-54, P3.58
Source
Recombinant Human ICAM1 /CD54 Protein, With C-Fc Tag (rhICAM1-Fc) Gln27-Glu480 (Accession # AAH15969) was produced in human HEK293 cells at ACRObiosystems.
Molecular Characterization
rhICAM1-Fc, fused with Fc fragment of human IgG1 at the C-terminus and has a calculated MW of 75.7 kDa expressed. The predicted N-terminus is Gln27. Protein migrates as 100-110 kDa in reduced SDS-PAGE resulting from glycosylation.
Endotoxin
Less than 1.0 EU per μg of the rhICAM1-Fc by the LAL method.
Purity
>98% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product format or formulation.
Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE
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The purity of rhICAM1-Fc was determined by SDS-PAGE of reduced rhICAM1-Fc and staining overnight with Coomassie Blue.
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5×104 cells/well are added to rhICAM/Fc Chimera coated plates (12.5 μg/ml with 100 μl/well), >60% will adhere after PMA 1 hour incubation at 37oC.
Background
Inter-Cellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes.[1] When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues.[2] ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies.[3-4] ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds.
References
(1) Rothlein, R. D., 1986, Journal of Immunology 137 (4): 1270–1274.
(2) Yang L, et al., 2005, Blood 106 (2): 584–92.
(3) Polin RS, et al., 1998, J. Neurosurg. 89 (4): 559–67.
(4) Frijns CJ, Kappelle LJ., 2002, Stroke 33 (8): 2115–22.
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