产品介绍
分子别名：
TNFRSF14, ATAR, HVEA, HVEM, LIGHTR, TR2, Tumor necrosis factor receptor superfamily member 14, herpesvirus entry mediator
表达区间及表达系统：
Recombinant Human HVEM /TNFRSF14 Protein, With C-Fc Tag (rhHVEM-Fc) Pro37-Val202 (Accession # NP_003811.2) was produced in human 293 cells (HEK293) at ACRObiosystems.
分子结构：
rhHVEM-Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 413 amino. rhHVEM-Fc, fused with the Fc region of human IgG1 at the C-terminus and has a calculated MW of 45.4 kDa expressed. Protein migrates as 50-60 kDa in reduced SDS-PAGE resulting from glycosylation.
内毒素：
Less than 1.0 EU per μg of the rhHVEM-Fc by the LAL method.
纯度：
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product format or formulation.
重构：
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
存贮：
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE:
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The purity of rhHVEM-Fc was determined by SDS-PAGE of reduced rhHVEM-Fc and staining overnight with Coomassie Blue.
活性：
Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L929 Mouse fibrosarcoma cells.
The ED50 for this effect is typically 2.5-10 μg/ml in the presence of 1 ng/ml of recombinant human TNF-beta.
产品背景：
Herpesvirus entry mediator (HVEM), also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily.
HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM.
Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.[1-4]
References
(1) Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
(2) Mauri, D.N. et al. (1998) Immunity 8:21
(3) Montgomery, R.I. et al. (1996) Cell 87:427
(4) Cheung,T.C.etal.,2005,Proc.Natl.Acad.Sci.USA102:13218-23
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