Synonym
HBsAg, adw
Source
Recombinant Hepatitis B Surface Antigen adw Subtype (rHBsAg-adw) was produced in Yeast cells.
Molecular Characterization
rHBsAg-adw full length monomeric protein contains 227 amino acids and the has a calculated MW of 24 kDa. Small amounts of dimer and trimer forms also exist DTT-reduced protein migrates as 24-27 kDa in SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg of the rHBsAg-adw by the LAL method.
Purity
>97% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 6.2 mM Phosphate, 200 mM NaCl bufer, pH 7.2. Normally Mannitol are added as protectants before lyophilization.
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Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8℃ for 1 year in lyophilized state
- 4-8℃ for 1 month under sterile conditions after reconstitution
- -20℃ to -70℃ for 3 months under sterile conditions after reconstitution
SDS-PAGE
![HBsAg]()
The purity of rHBsAg-adw was determined by SDS-PAGE of reduced rHBsAg-adw and staining overnight with Coomassie Blue.
Bioactivity
The activity of rHBsAg-adw protein was identified in a functional ELISA assay with anti-HBsAg-adw antibodies (capture and conjugate).
Background
Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. At the center of the hepatitis B virus is DNA, which contains the genes the virus uses to replicate itself. Surrounding the DNA is a protein called HBcAg (hepatitis B core antigen), which cannot be detected with blood tests. Surrounding this is HBsAg, which is actually part of the protective "envelope." This envelope surrounds the virus and protects it from attack by the body's immune system. HBsAg stands for hepatitis B surface antigen and is the surface antigen of the Hepatitis-B-Virus (HBV) S-gene. The capsid of a virus has different surface proteins from the rest of the virus. The antigen is a protein that binds specifically on one of these surface proteins. It is commonly referred to as the Australian Antigen. [1-2]
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References
- (1) Guarascio, P. et al., 1993, Journal of Clinical Pathology 36 (1): 18–23.
- (2) Blumberg B, Alter H., 1995, JAMA 191: 101–106.
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