产品介绍
Synonym
GM-CSF, CSF2, MGC131935
Source
Recombinant Human GM-CSF Protein, Native (rhGM-CSF) Ala18-Glu144, (Accession # NP_000749.2 ) was produced in human HEK293 cells at ACRObiosystems.
Molecular Characterization
rhGM-CSF, contains No Tag, has a calculated MW of 14.5 kDa. DTT reduced protein migrates as 16-26 kDa in SDS-PAGE resulting from different extent glycosylation.
Endotoxin
Less than 1.0 EU per μg of the rhGM-CSF by the LAL method.
Purity
>97% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product format or formulation.
Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE
![GM-CSF HEK293]()
The purity of rhGM-CSF was determined by SDS-PAGE of reduced rhGM-CSF and staining overnight with Coomassie Blue.
Bioactivity
The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells.
The ED50<0.1ng/ml, corresponding to a specific activity of >1X107 Unit/mg.
Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as Colony stimulating factor 2 (granulocyte-macrophage), is a cytokine initially characterized by its ability to induce colonies of granulocytes and macrophages from myeloid progenitor cells, and is secreted by macrophages, T cells, mast cells, endothelial cells and fibroblasts. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Monocytes exitthe circulation and migrate into tissue, whereupon they mature into macrophages and dendritic cells. Thus, it is part of the immune/inflammatory cascade, by which activation of a small number of macrophages can rapidly lead to an increase in their numbers, a process crucial for fighting infection. The active form of the protein is found extracellularly as a homodimer. Human GM-CSF glycosylated in its mature form. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising. GM-CSF is also used as a medication to stimulate the production of white blood cells following chemotherapy.[1-3].
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References
- (1) Volmar, C.H. et al., 2008, Cytokine. 42(3): 336-344.
- (2) Breitbach CJ, et al., 2010, Nature 477 (7362): 99–102.
- (3) Korzenik J, et al., 2005, N Engl J Med 352 (21): 2193–201.
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