产品介绍
Synonym
CSF3R, CD114, GCSFR, GCSF-R, G-CSF-R, granulocyte-colony-stimulating-factor-receptor
Source
Recombinant Human GCSFR /CD114 Protein, With C-Fc Tag (rhG-CSFR-Fc) Glu25-Pro621 (Accession # NP_000751.1) was produced in human 293 cells (HEK293) at ACRObiosystems.
Molecular Characterization
rhG-CSFR-Fc, fused with Fc region of human IgG1 at C-terminus and has a calculated MW of 92.8 kDa expressed. The predicted N-terminus is Glu 25. Protein migrates as 100-130 kDa in reduced SDS-PAGE resulting from glycosylation.
Endotoxin
Less than 1.0 EU per μg of the rhG-CSFR-Fc by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE
![GCSFR]()
The purity of rhG-CSFR-Fc was determined by SDS-PAGE of reduced rhG-CSFR-Fc and staining overnight with Coomassie Blue.
Bioactivity
Measured by its ability to inhibit the GCSF-induced proliferation of NFS-60 mouse myeloid cells.
The ED50 for this effect is typically 1-5 μg /ml in the presence of 0.125 ng /ml of recombinant human GCSF.
Background
Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as Cluster of Differentiation 114 (CD114), CSF3R and GCSF, is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF), a cytokine that plays a critical role in the regulation of the activation, proliferation, differentiation, and survival of the neutrophilic granulocyte lineage. G-CSFR belongs to a family of cytokine receptors known as the hematopoietin receptor family. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type I II (FNIII) domains in the extracellular region. G-CSFR is present mainly on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. G-CSFR mediates the specific effect of GCSF through activating a variety of intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways, and thus functions in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis.[1] Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.[2] Mutations in the intracellular part of this receptor are also associated with certain types of leukemia.[3]
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References
- (1) Fan Dong, Andrew C. Larner, 2000, BLOOD, VOLUME 95(5).
- (2) Zeidler C, Welte K, 2002, Semin. Hematol. 39 (2): 82–8.
- (3) Beekman R, Touw IP, 2010, Beekman R, Touw IP.
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