产品介绍
Synonym
FOLR2, FOLR-2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, Folate-receptor-beta
Source
Recombinant Human FOLR2 /FBP Protein (rhFOLR2) Thr17-His228 (Accession # P14207-1) was produced in human 293 cells (HEK293) at ACRObiosystems.
Molecular Characterization
rhFOLR2, fused with 6×his tag at the C-terminus and has a calculated MW of 26 kDa expressed. The predicted N-terminus is Thr17. Protein migrates as 30-33 kDa in reduced SDS-PAGE resulting from glycosylation.
Endotoxin
Less than 1.0 EU per μg of the rhFOLR2 by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE
![FOLR2]()
The purity of rhFOLR2 was determined by SDS-PAGE of reduced rhFOLR2 and staining overnight with Coomassie Blue.
Bioactivity
Measured by its binding ability in a functional ELISA. When Folic AcidBovine Serum Albumin was coated at 5 μg/ml (100 μl/well), the concentration of rhFOLR2 that produces 50% of the optimal binding response was found to be approximately 0.15-1.1 nM.
Background
Folate receptor beta, also known as Folate receptor 2, FBP, FOLR2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, and is a member of the folate receptor (FOLR) family. and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells. [1,3] It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers.[2,4] It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma. [5-6] FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages.
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References
- (1) Ratnam, M. et al., 1989, Biochemistry, 28(20):8249-54.
- (2) Ross, J.F. et al., 1999, Cancer, 85:348
- (3) Reddy, J.A. et al., 1999, Blood, 93:3940
- (4) Ross, J.F. et al.,1994, Cancer 73:2432
- (5) van der Heijden, J.W. et al., 2009, Arthritis Rheum. 60:12
- (6) Nagai, T. et al., 2009, Cancer Immunol. Immunother. Feb 24 epub
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