Synonym
FOLR-1, FBP, Folate Receptor Alpha
Source
Recombinant Mouse FOLR1 Protein(rmFOLR1) Thr25- Met231 (Accession# NP_032060.2)was produced in human 293 cells (HEK293) at ACRObiosystems.
Molecular Characterization
rmFOLR1, fused with 6×his tag at the C-terminus and has a calculated MW of 25.6 kDa expressed. The predicted N-terminus is Thr 25. Protein migrates as 45-50 kDa in reduced SDS-PAGE resulting from glycosylation.
Endotoxin
Less than 1.0 EU per μg of the rmFOLR1 by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).
SDS-PAGE
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The purity of rmFOLR1 was determined by SDS-PAGE of reduced rmFOLR1 and staining overnight with Coomassie Blue.
Bioactivity
Measured in a competitive binding assay. When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rmFOLR1 (7.9 nM)
At the concentration range of 10-100 nM.
Background
Folate Receptor 1 (FOLR1) also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage [1-3]. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas [4]. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface [3, 5]. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk [6]. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.[7]
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References
- (1) Lacey SW, et al., 1989, J. Clin. Invest. 84 (2): 715–20.
- (2) Elwood PC, 1989, J. Biol. Chem. 264 (25): 14893–901.
- (3) Rijnboutt, S. et al., 1996, J. Cell Biol. 132:35.
- (4) Parker, N. et al., 2005, Anal. Biochem. 338:284.
- (5) Paulos C M, et al., 2004, Mol Pharmacol. 2004;66:1406–1414.
- (6) Elwood, P.C. et al., 1991, J. Biol. Chem. 26:2346.
- (7) Dainty,L.A. et al., 2007, Gynecol Oncol. 105 (3):563-70.
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