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上海复蒙基因生物科技有限公司是美国Sciencell公司官网公布中国一级代理：

         美国ScienCell研究实验室（ www.sciencellonline.com ）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售8年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。 确保了实验结果的重复性和连贯性。

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 人支气管上皮细胞说明：
货号：3210
    

呼吸道上皮细胞是由纤毛、无纤毛和能分泌黏液的细胞按照从近端到远端呼吸道分布的混合细胞群组成。这类细胞的各个亚型细胞各具特点，从而不仅能形成物理屏障，可有效的防止多种有毒物质，而且通过产生和分泌大量化学介质和细胞因子形成高度复杂的宿主防御系统。支气管上皮由表面的上皮细胞和粘液腺体组成。表面的上皮细胞由基细胞，杯状细胞和纤毛细胞三种细胞构成，形成了基底柱状结构，并能清除粘液纤毛。用支气管上皮细胞培养分化诱导纤毛表型的研究显示，IL-4和IL-13的刺激能改变增殖、纤毛运动、黏液分泌等一系列细胞功能。支气管上皮细胞增殖以及其它细胞过程也部分受到EGF受体信号的调节。支气管上皮细胞的培养为我们研究这类细胞的功能和病理生理机制提供了一个体外模型。    人支气管上皮细胞(HBEC)提取于人肺组织，原代冻存。每管含有细胞数>5×10⁵cells/ml，此细胞通过Cytokeratin-18, -19和Vimentin抗体荧光免疫方法验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。
推荐培养基: (BECM, Cat. No. 3211)

DISCRIPTION

The respiratory epithelium is composed of a mixed population of ciliated, nonciliated, and mucous-secreting cells from proximal to distal airways. The individual characteristics of the subtypes of these cells create not only an effective physical barrier against various noxious substances, but also a highly sophisticated host defense system by producing and releasing a large number of chemical mediators and cytokines [1]. The bronchial epithelium consists of the surface epithelial cells and mucus glands. The surface epithelial cells are made up of three principle cell types: basal, goblet, and ciliated cells, of which the latter two form a suprabasal columnar structure and are necessary for mucociliary clearance. Studies using bronchial epithelial cell culture differentiated to induce a mucociliary phenotype have shown that IL-4 and IL-13 stimulation modulate basic cell functions such as proliferation, ciliary beating, and mucous production [2]. Cell proliferation, as well as various other cellular processes in bronchial epithelial cells, is also regulated in part by EGF receptor signaling [3]. The availability of bronchial epithelial cell culture provides an in vitro model to study the mechanisms of function and pathophysiology of these cells.
HBEpiC from ScienCell Research Laboratories are isolated from human lung tissue. HBEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HBEpiC are characterized by immunofluorescent method with antibodies CK-18, -19, and vimentin. HBEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBEpiC are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.
Reference
[1]. Velden, V. H., and H. F. Versnel. 1998. Bronchial epithelium: morphology, function and pathophysiology in asthma. Eur. Cytokine Netw. 9:585-597.
[2]. Kikuchi, T., Shively, J.D., Foley, J.S., Drazen, J.M., Tschumperlin, D.J. (2004) Differentiation-dependent responsiveness of bronchial epithelial cells to IL-4/13 stimulation. Am J Physiol Lung Cell Mol Physiol. 287:L119-26.
[3]. Kim S, Schein AJ, Nadel JA.(2005) E-cadherin promotes EGFR-mediated cell differentiation and MUC5AC mucin expression in cultured human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 289:L1049-60.