产品介绍
Mouse Monoclonal Antibody to HA-tag
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General Information
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Immunogen:
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HA peptide conjugated with KLH
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Clone ID:
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Mouse mAb
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Ig Type:
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IgG1
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Applications:
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WB , IHC , IP
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Specificity:
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Specifically detected HA tag fused protein.
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Formulation:
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0.2µm filtered solution in PBS with 50％ glycerol
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Storage:
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<-20℃
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with peptide YPYDVPDYA conjugated with KLH. The ascites was purified by ion exchange chromatography.
Applications
1) Western blot (1:1000) : This antibody can be used at 2μg with appropriate secondary reagents to detect HA-tagged proteins.
Lane 1, 2: 293T control
Lane 3, 4: 293T transfected with HA plasmid
M: protein ladder
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Immunoprecipitation (1:500)：This antibody can be used at 2μg to precipitate HA-tagged proteins.
Lane 1: HA mAb IP with 293T transfected with HA plasmid
Lane2: HA mAb IP with 293T transfected without HA plasmid
M: protein ladder
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Immunofluorescence (1:100): This antibody can be used at 2μg to detect HA-tagged proteins.
293T transfected with HA plasmid
1:100 dilution
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293T control，1:100 dilution
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Specificity
Mouse mAb specifically detects over-expressed proteins containing the HA epitope tag.
Storage
Store at -20℃, and stable for one year from the date of shipment.
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