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非小细胞肺癌肿瘤分子标志物检测试剂盒

 货号: BR-1101NL     规格: 96 Assay      价格: ￥3735.00

Non-small-cell lung carcinoma (NSCLC)  Molecular Marker (ALK and DAPK1) Detection Kit

 

Description: The kit provides a means of performing Non-small-cell lung carcinoma (NSCLC) detection method based on kinase activity assays. Three targets of molecular marker (DAPK1) are involved in NSCLC. This kit includes Streptavidin Coated Plates and specific Substrate-Peptides (biotinylated peptide substrates) for detection of the activity of DAPK1 which indicate the prognosis of NSCLC.

Molecular Markers in NSCLC

 

Disease	Molecular Marker	Gene ID	Gene Disease Relation
NSCLC	DAPK1	1612	marker/mechanism
NSCLC	DAPK1	1612	marker/mechanism

- Molecular markers for drug discovery and life science research (mechanism or therapeutic).

Peptide Core Sequence: 

Specific Substrate-Peptides for NSCLC Molecular Marker

 

Molecular Marker	Substrate-Peptide	Residue	Reaction Type
DAPK1	Peptides Mix	Ser-188	Phosphorylation
DAPK1	Peptides Mix	Ser-308	Phosphorylation

Label of Substrate-Peptides:  Biotinylated

Quality Control: The substrate peptide was selected using our NSCLC Detection Kit. The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.

Storage: 

Store at 4 °C, Avoid Freeze.

Background:

Death-associated protein kinase 1 (DAPK1) is an enzyme that in humans is encoded by the DAPK1 gene. Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.In melanocytic cells DAPK1 gene expression may be regulated by MITF. DAPK1 is a 16 kDa calmodulin-dependent serine/threonine kinase that carries a death domain at its C-terminus. DAP-kinase functions as a positive mediator of apoptosis that is induced by interferon-gamma. Recent studies suggest that DAP-kinase is involved in tumor metastasis and that it can be inactivated by methylation of CpG islands in the promoter region of the gene in some human tumors. It involves autophosphorylation of Ser(308) within the calmodulin (CaM)-regulatory domain, which occurs at basal state, in the absence of Ca(2+)/CaM, and is inversely correlated with substrate phosphorylation. This type of phosphorylation takes place in growing cells and is strongly reduced upon their exposure to the apoptotic stimulus of C(6)-ceramide. The substitution of Ser(308) to alanine, which mimics the ceramide-induced dephosphorylation at this site, increases Ca(2+)/CaM-independent substrate phosphorylation as well as binding and overall sensitivity of the kinase to CaM.

 

联系方式：

拜珐公司： 业务手机：18221844720   电话：021-34315602  邮箱：biopharmsoft@sina.com