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产品介绍

乳腺癌肿瘤分子标志物检测试剂盒

Breast Neoplasms Molecular Marker (ARAF, RAF1 and CHEK2) Detection Kit

Description: The kit provides a means of performing Breast Neoplasms detection method based on kinase activity assays. Three targets of molecular marker (ARAF, RAF1 and CHEK2) are involved in Breast Neoplasms. This kit includes Streptavidin Coated Plates and specific Substrate-Peptides (biotinylated peptide substrates) for detection of the activity of ARAF, RAF1 and CHEK2 which indicate the prognosis of Breast Neoplasms.

Molecular Markers in Breast Neoplasms

 

Disease	Molecular Marker	Gene ID	Gene Disease Relation
Breast Neoplasms	ARAF	369	therapeutic
Breast Neoplasms	RAF1	5894	therapeutic
Breast Neoplasms	CHEK2	11200	marker/mechanism

- Molecular markers for drug discovery and life science research (mechanism or therapeutic).

Peptide Core Sequence: 

Specific Substrate-Peptides for Breast Neoplasms Molecular Marker

 

Molecular Marker	Substrate-Peptide	Residue	Reaction Type
ARAF	Peptides Mix	Ser-218	Phosphorylation
RAF1	Peptides Mix	Ser-29	Phosphorylation
CHEK2	Peptides Mix	Ser-559	Phosphorylation

Label of Substrate-Peptides:  Biotinylated

Quality Control: The substrate peptide was selected using our Breast Neoplasms Detection Kit. The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.

Storage: 

Store at 4 °C, Avoid Freeze.

Background:

Serine/threonine-protein kinase A-Raf or simply A-Raf is an enzyme that in humans is encoded by the ARAF gene. A-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. RAF proto-oncogene serine/threonine-protein kinase also known as proto-oncogene c-RAF or simply c-Raf is an enzyme that in humans is encoded by the RAF1 gene. Raf-1 was shown to bind efficiently to Ras only when Ras is bound to GTP, not GDP.[9] In the MAPK/ERK pathway Raf-1 becomes activated when it binds to Ras. It is thought that phosphorylation of Raf-1 (at sites such as serine-338) upon binding of Raf-1 to Ras locks Raf-1 into an activated conformation that is then independent of binding to Ras for the continued activity of Raf-1. Several MAPK kinase kinase kinases have been suggested to be important for phosphorylation of Raf-1 as well as positive feedback phosphorylation by MAPK (ERK). CHEK2 is the official symbol for the human gene CHK2 checkpoint homolog. It is located on the long (q) arm of chromosome 22. A deletion-mutation at position 1100 of the CHEK2 gene is associated with an increased risk of breast cancer, particularly in the European population. In women of Northern and Eastern European descent CHEK2*1100delC carrier status confers a 2-3 fold risk of breast cancer. In this ethnic group, women with a familial history of breast cancer have a 4.8 fold risk of breast cancer equal to a lifetime risk of breast cancer of 37%.

 

 

联系方式：

拜珐公司： 业务手机：18221844720   电话：021-34315602  邮箱：biopharmsoft@sina.com