OmicsLink?ORF表达克隆（编码全长蛋白的ORF表达克隆)_嘉美诺斯生物科技（北京）有限公司

OmicsLink?ORF表达克隆（编码全长蛋白的ORF表达克隆)

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产地：北京
关注度：次
品牌：无
型号：欢迎咨询！
厂商性质：生产型,贸易型,
价格：电议
添加时间： 2011/11/9

产品介绍

在人类基因组测序计划的完成之后，大规模的人类蛋白功能研究和阐明便成为了当今生物医学和制药领域迅速发展的限速步骤。OmicsLink™ ORF 表达克隆系列产品为研究人员提供了70多套各20,000多个高质量的人类基因全长ORF cDNA克隆。 OmicsLink™ ORF 表达克隆可直接用于蛋白表达，生产和纯化，定位及其他多种蛋白功能研究。OmicsLink™ ORF表达克隆在基因组学，蛋白质组学和生物医学以及药物研究和开发领域之间起着重要的纽带和连结作用。

不同的OmicsLink^™ ORF Expression Clones，可立即用于在细菌，酵母，昆虫和人类

装载ORF表达克隆的OmicsLink^™表达载体系列

Human and Mouse Full Length ORF Wheat Germ Cell Free Expression Clones (8 types)

Vector	Promoter	Host Cell	Selecting Marker	Tag	Protease Site
pReceiver-WG02	T7	cell free	N/A	N-His	Factor Xa
pReceiver-WG03	T7	cell free	N/A	N-HisSUMO	CoolCutter(TM)
pReceiver-WG04	T7	cell free	N/A	N-AviSUMO	CoolCutter(TM)
pReceiver-WG05	T7	cell free	N/A	N-HisAviSUMO	CoolCutter(TM)
pReceiver-WG09	T7	cell free	N/A	HisGST	TEV
pReceiver-WG16	T7	cell free	N/A	N/A	N/A
pReceiver-WG31	T7	cell free	N/A	N-HisSUMOAvi	CoolCutter(TM)
pReceiver-WG33	T7	cell free	N/A	N-TrxHisSUMO	CoolCutter(TM)

Human and Mouse Full Length ORFEXPRESS™ Gateway® Shuttle Clone(Gateway® pDEST compatible)

Vector	Promoter	Host Cell	Selecting Marker	Tag	Protease Site
pDONR™ Vector	ORFEXPRESS™ Shuttle Clone (Gateway® Entry Clone)	N/A	N/A	N/A	N/A

Human and Mouse Expression Ready Bacterial Full Length ORF cDNA Clones (14 types)

Vector	Promoter	Host Cell	Selecting Marker	Tag	Protease Site
pReceiver-B01	T7	E. Coli	N/A	N-His	N/A
pReceiver-B31	T7	E. Coli	N/A	C-His	N/A
pReceiver-B02	T7	E. Coli	N/A	N/A	N/A
pReceiver-B03	T7	E. Coli	N/A	N-GST	Tev
pReceiver-B04	T7	E. Coli	N/A	N-GST	EK
pReceiver-B05	Tac	E. Coli	N/A	N-GST	Tev
pReceiver-B06	Tac	E. Coli	N/A	N-GST	EK
pReceiver-B07	Tac	E. Coli	N/A	N-MBP	Tev
pReceiver-B08	Tac	E. Coli	N/A	N-MBP	EK
pReceiver-B09	T7	E. Coli	N/A	N-Avi	N/A
pReceiver-B10	Tac	E. Coli	N/A	N-Flag	N/A
pReceiver-B11	Tac	E. Coli	N/A	N-His	N/A
pReceiver-B12	Tac	E. Coli	N/A	HisSUMO	Sumo protease
pReceiver-B13	T7	E. Coli	N/A	HisSUMO	SUMO Protease

Human and Mouse Expression Ready Mammalian Full Length ORF cDNA Clones (31 types)

Vector	Promoter	Host Cell	Selecting Marker	Tag	Protease Site
pReceiver-M01	CMV	Mammalian	Neomycin	N-His	N/A
pReceiver-M02	CMV	Mammalian	Neomycin	N/A	N/A
pReceiver-M29	CMV	Mammalian	Neomycin	N-eGFP	N/A
pReceiver-M03	CMV	Mammalian	Neomycin	C-eGFP	N/A
pReceiver-M15	CMV	Mammalian	Neomycin	N-eYFP	N/A
pReceiver-M16	CMV	Mammalian	Neomycin	C-eYFP	N/A
pReceiver-M32	CMV	Mammalian	Neomycin	N-eCFP	N/A
pReceiver-M33	CMV	Mammalian	Neomycin	C-eCFP	N/A
pReceiver-M04	CMV	Mammalian	Neomycin	N-GST	EK
pReceiver-M05	CMV	Mammalian	Neomycin	N-Avi	N/A
pReceiver-M48	CMV	Mammalian	Neomycin	N-Avi+ IRES-Biotin ligase	N/A
pReceiver-M17	CMV	Mammalian	Neomycin	C-Avi	N/A
pReceiver-M06	CMV	Mammalian	Neomycin	N-3xHA	N/A
pReceiver-M07	CMV	Mammalian	Neomycin	C-3xHA	N/A
pReceiver-M08	CMV	Mammalian	Neomycin	C-3xHA-His	N/A
pReceiver-M45	CMV	Mammalian	Neomycin	C-3xHA+ IRES-eGFP	N/A
pReceiver-M09	CMV	Mammalian	Neomycin	C-Myc	N/A
pReceiver-M10	CMV	Mammalian	Neomycin	C-Myc-His	N/A
pReceiver-M47	CMV	Mammalian	Neomycin	C-Myc+ IRES-eGFP	N/A
pReceiver-M11	CMV	Mammalian	Neomycin	N-Flag	N/A
pReceiver-M12	CMV	Mammalian	Neomycin	N-3XFlag	N/A
pReceiver-M13	CMV	Mammalian	Neomycin	C-Flag	N/A
pReceiver-M46	CMV	Mammalian	Neomycin	C-Flag+ IRES-eGFP	N/A
pReceiver-M14	CMV	Mammalian	Neomycin	C-3XFlag	N/A
pReceiver-M24	CMV	Mammalian	Neomycin	N-SNAP	N/A
pReceiver-M26	CMV	Mammalian	Neomycin	C-SNAP	N/A
pReceiver-M49	CMV	Mammalian	Neomycin	N-HaloTag	Tev protease
pReceiver-M50	CMV	Mammalian	Neomycin	C-HaloTag	Tev protease
pReceiver-M55	CMV	Mammalian	Neomycin	N-mCherry	N/A
pReceiver-M56	CMV	Mammalian	Neomycin	C-mCherry	N/A
pReceiver-M60	CMV	Mammalian	Neomycin	IRES-eGFP	N/A

Human and Mouse Expression Ready Lentiviral Full Length ORF cDNA Clones (39 types)

Vector	Promoter	Host Cell	Selecting Marker	Tag	Protease Site
pReceiver-Lv01	CMV	Stem/primary cell	No	N/A	N/A
pReceiver-Lv31	CMV	Stem/primary cell	No	IRES-eGFP	N/A
pReceiver-Lv36	CMV	Stem/primary cell	No	+ IRES-luciferase	N/A
pReceiver-Lv40	CMV	Stem/primary cell	Neomycin	IRES-Neomycin	N/A
pReceiver-Lv21	CMV	Stem/primary cell	Neomycin	N/A	N/A
pReceiver-Lv02	CMV	Stem/primary cell	No	C-3xHA	N/A
pReceiver-Lv32	CMV	Stem/primary cell	No	C-3xHA + IRES-eGFP	N/A
pReceiver-Lv52	CMV	Stem/primary cell	N/A	C-3xHA+IRES2-eGFP	N/A
pReceiver-Lv06	CMV	Stem/primary cell	Neomycin	C-3xHA	N/A
pReceiver-Lv23	CMV	Stem/primary cell	Neomycin	N-Flag	N/A
pReceiver-Lv03	CMV	Stem/primary cell	No	C-Flag	N/A
pReceiver-Lv33	CMV	Stem/primary cell	No	C-Flag + IRES-eGFP	N/A
pReceiver-Lv53	CMV	Stem/primary cell	N/A	C-Flag+IRES2-eGFP	N/A
pReceiver-Lv07	CMV	Stem/primary cell	Neomycin	C-Flag	N/A
pReceiver-Lv19	CMV	Stem/primary cell	Neomycin	N-eGFP	N/A
pReceiver-Lv04	CMV	Stem/primary cell	No	C-eGFP	N/A
pReceiver-Lv08	CMV	Stem/primary cell	Neomycin	C-eGFP	N/A
pReceiver-Lv20	CMV	Stem/primary cell	Neomycin	N-eYFP	N/A
pReceiver-Lv05	CMV	Stem/primary cell	No	C-eYFP	N/A
pReceiver-Lv09	CMV	Stem/primary cell	Neomycin	C-eYFP	N/A
pReceiver-Lv34	CMV	Stem/primary cell	Neomycin	N-eCFP	N/A
pReceiver-Lv61	CMV	Stem/primary cell	No	C-eCFP	N/A
pReceiver-Lv62	CMV	Stem/primary cell	Neomycin	C-eCFP	N/A
pReceiver-Lv35	CMV	Stem/primary cell	No	N-Avi + IRES-Biotin ligase	N/A
pReceiver-Lv26	CMV	Stem/primary cell	Neomycin	N-Avi	N/A
pReceiver-Lv10	CMV	Stem/primary cell	Neomycin	C-Avi	N/A
pReceiver-Lv25	CMV	Stem/primary cell	Neomycin	N-Myc	N/A
pReceiver-Lv17	CMV	Stem/primary cell	Neomycin	C-Myc	N/A
pReceiver-Lv18	CMV	Stem/primary cell	Neomycin	C-Myc-His	N/A
pReceiver-Lv43	CMV	Stem/primary cell	No	C-Myc+ IRES-eGFP	N/A
pReceiver-Lv70	CMV	Stem/primary cell	N/A	C-Myc+IRES2-eGFP	N/A
pReceiver-Lv44	CMV	Stem/primary cell	No	C-Myc+ IRES-eYFP	N/A
pReceiver-Lv47	CMV	Stem/primary cell	Neomycin	C-Myc+IRES-Neomycin	N/A
pReceiver-Lv64	CMV	Stem/primary cell	Neomycin	N-HaloTag	Tev protease
pReceiver-Lv65	CMV	Stem/primary cell	Neomycin	C-HaloTag	Tev protease
pReceiver-Lv69	CMV	Stem/primary cell	N/A	C-Myc+IRES-mCherry	N/A
pReceiver-Lv71	CMV	Stem/primary cell	Puromycin	N-mCherry	N/A
pReceiver-Lv72	CMV	Stem/primary cell	Neomycin	C-mCherry	N/A
pReceiver-Lv41	EF1a	Stem/primary cell	Neomycin	N/A	N/A

Human and Mouse Expression Ready Yeast Full Length ORF cDNA Clones (3 types)

Vector	Promoter	Host Cell	Selecting Marker	Tag	Protease Site
pReceiver-Y01	GAL1	S. cerevisiae	N/A	C-His	N/A
pReceiver-YAD	pADH	Yeast	N/A	GAL4AD	N/A
pReceiver-YBD	pADH	Yeast	N/A	GAL4DB	N/A

Human and Mouse Expression Ready Insect Full Length ORF cDNA Clones (1 type)

Vector	Promoter	Host Cell	Selecting Marker	Tag	Protease Site
pReceiver-I01	AcMNPV polyhedrin	Insect cell	N/A	N-His	Tev

可用于体内或体外表达

通过简单的“点击－选择－订购”，你便可以立刻用OmicsLink™ORF表达克隆表达你所需要的蛋白并对其进行各类的功能研究。OmicsLink™ORF表达克隆系列产品是构建在70多种带有不同启动子和标签融合蛋白表达载体里。载体中的优化的翻译信号和多种特异标签蛋白使得这些表达克隆特别地适合于蛋白表达，纯化，定位以及其他各类的功能研究。本公司还提供OmicsMate™ 客户特定产品制作和技术咨询服务，构建客户需要但现在不在我们提供的产品目录中的特制克隆。

OmicsLink^™ ORF 表达克隆在系统生物学和整合生物学的应用

在E. coli中表达重组蛋白
用反向转染技术在哺乳动物细胞中表达蛋白和细胞定位
在体外无细胞翻译系统中高通量蛋白生产
表达有特异标记的蛋白，用于分离，纯化和固相化及下游功能研究和分析
生产用于组织或器官基因表达谱分析的原位杂交的探针
生产用于基因沉默功能分析的esiRNA模板

OmicsLink^™ ORF表达克隆和全长cDNA克隆的区别

Clone Characteristics	OmicsLink™ ORF clones	Whole Transcript cDNA Clones
Gene transcript	ORF (Start to stop only)	Whole transcripts including 5' and 3' UTRs
Expression ready	Yes	Some are, most will need further subcloning procedures to remove 5' and 3' UTRs
Mammalian cell transfection - Transicent Transfection - Stable Transfection	Yes Yes	Only a small number No
Unique protein tags - Protein-Molecule interactions - Concentration of lowly expressed proteins - Affinity purification of expressed proteins	Yes Yes Yes Yes	No No No No
shRNAi validation and rescue	Yes	No
miRNA and miRNA target validation and rescue	Yes	No
*Produce proteins via in vitro* cell-free translation systems**	Yes	No
production Template for esiRNA	Yes	No
*Probe generations for in situ* hybridization**	Yes	No

质量保证

OmicsLink™ORF表达克隆用经过严格验证的人类全长cDNA克隆和高质量的人体组织cDNA文库作PCR模板构建的。这些高质量OmicsLink™ORF表达克隆在投递前都经过严格的多种QC/QA过程的检测。

* 本产品仅供研究、不用于临床诊断

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