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产品介绍

产品名称:Easy Yeast Plasmid Isolation Kit
产品价格:2075
产    地:Clontech
产品货号:630467
产品规格:50 Preps
产品说明:
Features
?Rescue plasmids from yeast using spin columns
?Highly efficient Zymolyase is included
?Purify DNA in less time
Applications
?Plasmid DNA mini-preps from yeast
?Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen
The Easy Yeast Plasmid Isolation Kit provides a simple and efficient plasmid mini-prep method for rescuing plasmid DNA from yeast (Saccharomyces cerevisiae). The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.