产品介绍
产品名称:Fusion-Blue Competent Cells
产品价格:4332
产 地:Clontech
产品货号:636758
产品规格:5*20 μL
产品说明:
Features
Stellar Competent Cells
Transformation Efficiency: > 5 × 108 cfu/μg
Stellar Competent Cells (dam–/dcm–)
Transformation Efficiency: > 1 × 106 cfu/μg
Applications
?Cloning, subcloning
?Methylated DNA cloning
?cDNA and genomic library construction
?Longer-length cDNA library construction
An E. coli HST08 strain that provides high transformation efficiency. These cells can be used in a wide variety of applications—from preparation of cDNA and genomic libraries, to construction of longer-length genomic libraries, subcloning, and even methylated DNA cloning. Stellar Competent Cells lack the gene cluster for cutting foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning methylated DNA. The cells can also be used for blue/white screening (i.e., alpha-complementation) when transformed with vectors containing the lacZalpha gene. Stellar Competent Cells are ideal for use with Clontech's In-Fusion Cloning Kits.