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Description:

Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240 kDa and containg 4 subunits each with a binding site for biotin. The Streptavidin is isolated by purification from fermentation broth.
The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react unspecifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.

Physical Appearance:

Sterile Filtered lyophilized powder.

Formulation:

1mM MgCl2, 0.1mM ZnCl2, 30 mM triethanolamine pH 7.6.

Solubility:

It is recommended to reconstitute the lyophilized Streptavidin in sterile 18MΩ-cm H²O + 0.1M NaCl not less than 100µg/ml or more than 10mg/ml solutions.

Stability:

Streptavidin although stable at 4⁰C for 3 weeks, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Application:

Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.

Activity:

Greater than 13 U/mg, 1 unit binds 1µg biotin.

Endotoxin:

Less than 0.1 ng/µg (IEU/µg) of Streptavidin.

Usage:

Prospecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.