产品介绍
KAPA 高保真热启动酶
KAPA高保真热启动酶(KAPAHiFi™ HotStart)是二代基因工程酶,保真性强,活力超高。基因工程改造后的KAPA高保真热启动酶，可以提高扩增产物的产量、酶的灵敏度、扩增速度、目标片段长度，扩增复杂模板的能力。
一般的高保真酶扩增1kb碱基大约需要1-2分钟，而KAPA高保真热启动酶只需要30秒，可以节省75%的实验时间。KAPA高保真热启动酶具有很强的3’-5’外切酶，确保扩增准确性。KAPA高保真热启动酶，含有2种buffer，分别用于常规模板扩增和高难度模板扩增。
具有以下特点:
1.超级保真;
2.可以扩增各种复杂模板;
3.扩增长度长,扩增基因组可超过15kb;
4.扩增速度快,节省75%以上的时间。
应用范围：
1.定点突变
2.测序
3.蛋白表达。
一、超级保真性
图1.KAPA高保真酶扩增的出错率是野生型Taq酶的1/30，是Taq blends的1/10，是野生型Pfu的1/3。
二、扩增产量高、灵敏性高
Figure 2. Amplification of hgDNA targets up to 11 kb. Each target was amplified from a descending range of template hgDNA concentrations (50 ng to 0.5 ng per reaction). Reactions (25 μl each) were performed using standard 3-step cycling profiles (35 cycles): 20 sec denaturation, 15 sec annealing, and 30 sec/kb extension time. Total reaction time for the 11 kb amplicon was 3h 50mins. 12.5 μl of each reaction was loaded on the gel.
三、超级灵敏、高度保真
Figure3. 2 kb lambda phage target amplified from a 10 fold dilution series starting from 10 ng down to 10 fg using KAPAHiFi™ HotStart DNA Polymerase with Fidelity Buffer, fusion technology polymerase, and polymerase blend systems. All reactions (25 μl each) were performed using manufacturers’ protocols and buffers, with standard 3-step cycling profiles (35 cycles). 12.5 μl of each reaction was loaded on the gel.
四、困难复杂模板的扩增
Figure 4. Amplification of AT- and GC-rich, single-copy human genomic targets. Seven single-copy gene fragments representing a range of GC content were used to compare the robustness of KAPAHiFi™ HotStart DNA Polymerase against a panel of competitor high fidelity DNA polymerases and polymerase blends. Amplicons range between 0.5 kb and 0.7 kb in length and ranged from 47% to 84% GC content. All reactions (25 μl each) contained 50 ng human genomic DNA as template and were performed using manufacturers’ protocols and buffers, with standard 3-step cycling profiles (35 cycles). 12.5 μl of each reaction was loaded on the gel.
五、产品信息
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产品编号
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产品名称
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产品描述
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产品规格
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KK2101
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HiFi
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High fidelity
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100 units,200reactions
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KK2102
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HiFi
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High fidelity
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250 units,500reactions
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KK2501
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HiFi Hot Start
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High fidelity
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100 units,200reactions
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KK2502
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HiFi Hot Start
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High fidelity
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250 units,500reactions
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KK2601
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HiFi Hot Start
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Readymix (100 x 25 ul rxn)
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100 rxn
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KK2602
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HiFi Hot Start
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Readymix (500 x 25 ul rxn)
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500 rxn
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