产品介绍
Beckman A63880/A63881 Agencourt AMPure XP -核酸纯化试剂盒
现货beckman A63880/A63881/A63882 PCR纯化系统 Agencourt AMPure XP - PCR Purification 订货电话 18754115463
Product | Size | Kit Components | Product # |
---|
Agencourt AMPure XP 5 mL Kit | 139/278 preps1 | Agencourt AMPure XP Reagent | A63880 |
Agencourt AMPure XP 60 mL Kit | 1,666/3,333 preps1 | Agencourt AMPure XP Reagent | A63881 |
Agencourt AMPure XP 450 mL Kit | 12,500/25,000 preps1 | Agencourt AMPure XP Reagent | A63882 |
Application: PCR purification
Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking
Agencourt AMPure XP Features
High recovery of amplicons, greater than 100 bp
Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
Efficient recovery of double stranded and single stranded DNA templates
Consistent recovery throughout the kit’s 12 month shelf life
Faster manual and automated processing as compared to traditional post-PCR cleanup methods
Process Overview
1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate
High Recovery
Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).
Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.
Walk-away Automated Solution
Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NXP and FXP for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.
Ordering Information【济南翔宇生物科技有限公司 电话18754115463 】
Beckman A63880/A63881 Agencourt AMPure XP -核酸纯化试剂盒
Beckman A63880/A63881 Agencourt AMPure XP -核酸纯化试剂盒