货号: 26003 Gαi 小鼠单抗 |
| 基因符号: Gnai |
| 描述: Anti-Gαi Mouse Monoclonal Antibody |
| 背景介绍: Heterotrimeric G proteins are critical cellular signal transducers. Gai represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gai proteins are being explored. |
| 免疫原: Recombinant full length protein of Gαi1 |
| 经过测试的应用: ELISA, WB, IHC |
推荐稀释比:
ELISA 1: 1000-1: 5000,
WB 1: 1000-1: 2000,
IHC 1: 100-1: 200 |
| 浓度: 1 mg/ml |
| 宿主: mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Gαi antibody recognizes Gαi1, Gαi2, and Gαi3 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:  Western blot analysis of Gi proteins. Gαi1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blots. The monoclonal anti-Gαi antibody was pre-incubated without (lane 1) or with (lane 2) the antigen protein (Gαi1) to show the specificity. |
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