货号: 26076 TP53(R282W) 小鼠单抗 |
| 基因符号: TP53 |
| 描述: Anti-TP53(R282W) Mouse Monoclonal Antibody |
| 背景介绍: p53 protein acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are found in many types of cancers. |
| 免疫原: Recombinant p53(R282W) protein, human origin. |
| 经过测试的应用: ELISA, WB, IHC, IP |
推荐稀释比:
ELISA: 1: 1000-1: 5000
WB: 1: 1000-1: 5000
IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG1 |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-TP53(R282W) antibody recognizes TP53(R282W) of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: 
Western blot analysis of recombinant TP53(R282W) and wild-type p53 proteins. Purified His-tagged TP53(R282W) protein (lane 2, Cat.#10204) and wild type p53 protein (lane 1, Cat.#10202) were blotted with anti TP53(R282W) mouse monoclonal antibody (Cat. # 26076). 免疫荧光: 
Immunofluorescence of cells* TP53 proteins with anti-TP53 (R282W) antibody. HEK293T cells were transfected with pCDNA3-GFP-TP53 (WT) plasmid (left column) or pCDNA3-GFP-TP53(R282W) plasmid (right column), then fixed and stained with anti- TP53 (R282W) monoclonal antibody (Cat. # 26076). |
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