来宝网 2013/8/13点击2086次
pLVX-IRES-ZsGreen1载体
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VMC0380 | pLVX-IRES-ZsGreen1 | Clontech | 慢病毒载体 |
下载地址：http://www.chem17.com/st178406/Info_105643.html
Description
pLVX-IRES-ZsGreen1 is an HIV-1-based, lentiviral expression vector that allows the simultaneous
expression of your protein of interest and ZsGreen1 in virtually any mammalian cell type,
including primary cells. ZsGreen1 is a human codon-optimized variant of the reef coral
Zoanthus sp. green fluorescent protein, ZsGreen. The vector expresses the two proteins from
a bicistronic mRNA transcript, allowing ZsGreen1 to be used as an indicator of transduction
efficiency and a marker for selection by flow cytometry.
Expression of the bicistronic transcript is driven by the constitutively active human
cytomegalovirus immediate early promoter （P
CMV IE） located just upstream of the MCS. An
encephalomyocarditis virus （EMCV） internal ribosome entry site （IRES）, positioned between
the MCS and ZsGreen1, facilitates cap-independent translation of ZsGreen1 from an internal
start site at the IRES/ZsGreen1 junction （1）
