来宝网 2013/8/13点击1615次
型号 | 载体名称 | 出品公司 | 载体用途 |
VMC0381 | pLVX-AcGFP1-N1 | Clontech | 慢病毒载体 |
pLVX-AcGFP1-N1 is an HIV-1-based, lentiviral expression vector that allows you to express
your gene of interest fused to AcGFP1, a green ? uorescent protein derived from Aequorea
coerulescens. Genes cloned into the multiple cloning site (MCS), located upstream of the
AcGFP1 coding sequence, are expressed as N-terminal fusions of the AcGFP1 protein.
Expression of the fusion protein is driven by the constitutively active human cytomegalovirus
immediate early promoter (P
CMV IE) located just upstream of the MCS. Lentiviral particles
derived from the vector allow the expression of AcGFP1 fusion proteins in virtually any cell
type, including primary cells. The unmodi? ed vector expresses AcGFP1, and may be used to
produce marker virus to optimize infection protocols.