来宝网 2013/8/13点击1371次
型号 | 载体名称 | 出品公司 | 载体用途 |
VMA0372 | pSicoR | Addgene | 慢病毒载体 |
Backbone size (bp): 7567
Modifications to Backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5’: HpaI
Cloning site 3’: XhoI
5’ sequencing primer: mU6-F
pSicoR allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.