来宝网 2013/7/30点击2656次
pGBKT7-53
Bacteria Resistance：KanamycinMammalian Selection：-
载体特点：与pGADT7-T构成Matchmaker Two-Hybrid System 3的双杂交正对照。
pGBKT7-53 encodes a fusion of the murine p53 protein （a.a. 72–390） and the GAL4 DNA-BD （a.a. 1–147）. The murine p53 cDNA （GenBank Accession #K01700） was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. （1993）; plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.
pGBKT7-Lam
Bacteria Resistance：KanamycinMammalian Selection：-
载体特点：Matchmaker Two-Hybrid System 3的负对照。
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein （a.a. 66–230） and the GAL4 DNA-BD （a.a. 1–147）. The lamin C cDNA insert （GenBank Accession #M13451） was derived from the plasmid referenced in Bartel et al. （1993a）. Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
