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a)DiI和DiO = Omega XF52,Chroma 51004
b)DiI和DiD = Omega XF92,Chroma 51007
c)DiI,DiO和DiD = Omega XF93,Chroma 61005
5.流式细胞仪检测:
用DiO,DiI,DiD,DiS和DiR标记的细胞可分别使用常规FL1,FL2,FL3和FL4流式细胞仪检测通道进行分析。
参考文献
1. Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ. (2006) Confocal laser scanning microscopy using dialkylcarbocyanine dyes for celltracing in hard and soft biomaterials. J Biomed Mater Res B Appl Biomater.
2. Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K. (2006) In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope. Invest Ophthalmol Vis Sci, 47, 2943.
3. Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A. (2006) Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. J Biomed Opt, 11, 050507.
4. Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C. (2006) Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius. Hypertension, 48, 482.
以上是这几种细胞膜荧光探针的染色方法,与君共勉!