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下列发表的文章采用美国Sage Science公司的Pippin Prep和Blue Pippin 全自动核酸电泳和片段回收系统或者从采用其技术或材料：
环亚生物科技有限公司（APG BIO Ltd）是美国Sage Science公司在中国大陆的总代理商，详情请登录www.apgbio.com
October 2013
Semiconductor-based DNA sequencing of histone modification states
Authors:
Christine S. Cheng, Kunal Rai, Manuel Garber, Andrew Hollinger, Dana Robbins, Scott Anderson, Alyssa Macbeth, Austin Tzou, Mauricio O. Carneiro, Raktima Raychowdhury, Carsten Russ, Nir Hacohen, Jeffrey E. Gershenwald, Niall Lennon, Chad Nusbaum, Lynda Chin, Aviv Regev & Ido Amit
Info:
Researchers at the Broad Institute and collaborators at several institutions provide optimized sample preparation protocols for the generation of ChIP-seq libraries on the Ion Torrent PGM. They showed that Pippin size selection was required to generate usable libraries, even down to sub-nanogram input. Results were comparable to results from Illumina Chip-Seq workflow.
Citation:
Nature Communications 4:2672
http://dx.doi.org/10.1038/ncomms3672
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October 2013
Mitochondrial Sequence Variation in African-American Primary Open-Angle Glaucoma Patients (Pippin Prep)
Authors:
David W. Collins, Harini V. Gudiseva, Benjamin T. Trachtman, Matthew Jerrehian, Thomasine Gorry, William T. Merritt III, Allison L. Rhodes, Prithvi S. Sankar, Meredith Regina, Eydie Miller-Ellis, Joan M.
O’Brien
Info:
Researchers at the University of Pennsylvania deep-sequenced mitochondrial DNA of patients with primary open-angle glaucoma, plus a control group for comparison. They testing a theory implicating variation in mtDNA in this disease, but it could not be confirmed. Pippin Prep was used for size selection of Ion PGM libraries, per the Ion Express protocol.
Citation:PLoS ONE 8(10): e76627
http://dx.doi.org/10.1371/journal.pone.0076627
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October 2013
Genetic Basis for the Biosynthesis of the Pharmaceutically important Class of Epoxyketone Proteasome Inhibitors
Authors:
Michelle Schorn, Judith Zettler, Joseph P. Noel, Pieter C. Dorrestein, Bradley S. Moore, and Leonard Kaysser
Info:
Scientists from California and Germany teamed up to study genes that encode for the biosynthesis of an important component of certain proteasome inhibitors used for cancer treatment. The Ion PGM was used to sequence gene clusters in two microbial strains, reporting the first successful characterization of a region producing natural peptidyl-epoxyketones. For this project, libraries were prepared from genomic DNA sheared to 100 bp to 250 bp, after which sequencing material was separated and extracted using Pippin Prep.
Citation:ACS Chem. Biol. October 2013
http://dx.doi.org/10.1021/cb400699p
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October 2013
Improved Protocols for Illumina Sequencing
Authors:
Iraad F. Bronner, Michael A. Quail, Daniel J. Turner, and Harold Swerdlow
Info:
A comprehensive overview of the state-of-the state of Illumina sequencing, from the Wellcome Trust Sanger Institute and Oxford Nanopore Technologies. The value of narrow size distribution and Pippin automated size selection is discussed.
Citation:
Current Protocols in Human Genetics 18.2.1-18.2.42, October 2013
http://dx.doi.org/10.1002/0471142905.hg1802s79
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September 2013
Next-Generation Sequence Analysis of Cancer Xenograft Models
Authors:
Fernando J. Rossello, Richard W. Tothill, Kara Britt, Kieren D. Marini, Jeanette Falzon, David M. Thomas, Craig D. Peacock, Luigi Marchionni, Jason Li, Samara Bennett, Erwin Tantoso, Tracey Brown, Philip Chan, Luciano G. Martelotto, D. Neil Watkins
Info:
The authors looked at using xenografts as preclinical models for NGS cancer studies. The authors were able to show that mouse specific sequence was easily distinguished from human small cell lung cancer sequences. Pippin Prep size selection was used for whole genome library construction for the Illumina HiSeq.
Citation:PLoS ONE 8(9): e74432
http://dx.doi.org/10.1371/journal.pone.0074432
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August 2013
Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway
Authors:
Tao-Yi Cai, Wei Zhu, Xiong-Sheng Chen, Sheng-Yuan Zhou, Lian-Shun Jia and Yan-Qing Sun
Department of Orthopedic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China
Info:
From the Second Military Medical University in Shanghai, China, this qPCR gene expression study looks at the effect of fibroblast growth factor mesenchymal stems cells. The BluePippin was used to size-selec cDNAs.
Citation:Molecular Medicine Reports 8: 1323-1328, 2013
