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 Human Tumor Necrosis Factor α (TNF-α) ELISA Kit

Catalog No. CSB-E04740h

(96 T)

?          This immunoassay kit allows for the in vitro quantitative determination of human TNF-α concentrations in serum, plasma and other biological fluids.

?          Expiration date six months from the date of manufacture

?          FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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INTRODUCTION

The prototype ligand of the TNF superfamily, TNF-α/TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis. It is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNF-αis assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNF-α (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNF-α-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Human cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3+CD56+ hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4+ and CD8+ T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

PRINCIPLE OF THE ASSAY

The microtiter plate provided in this kit has been pre-coated with

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