电穿孔(Electroporation ), 也叫电转染。是功能强大的将核酸、蛋白及其它分子导入多种细胞的高效技术。通过高强度的电场作用，瞬时提高细胞膜的通透性，从而吸收周围介质中的外源分子。这种技术可以将核苷酸、DNA 与RNA、蛋白、糖类、染料及病毒颗粒等导入原核和真核细胞内。电转化相对其它物理和化学转化方法，是一种有价值和有效的替代方法

 

瑞士Cellectricon  Cellaxess ACE贴壁细胞电穿孔仪，在原位转染贴壁原代细胞和iPSC衍生细胞

(Cellectricon  Cellaxess ACE Adherent Cell Electroporation,In-situ Transfection of Adherent Primary and iPSC-derived Cells

)

 

该系统显著特点是：

 

Ø 全面的灵活性 – 适用于任何类型的细胞，任何基材(Perfectly retained cell function and morphology)

Ø 完美保留了细胞的功能和形态(Complete flexibility – any cell type, any substrate)

Ø 高效且易于使用 (Efficient and easy to use)

 

 

 

 

 

In-situ transfection of adherent primary and iPSC? derived cells at any developmental stage

原位转染任何发展阶段的贴壁原代和iPSC衍生细胞

 

Cellaxess electroporation gives you superior and dependable in-plate transfection as low voltages and no cell processing are required.

With Cellaxess ACE, cells can be transfected at any stage of development with no impact on cell health or morphology. Cellaxess ACE enables in-situ  transfection of any primary

and iPSC-derived cell type in its adherent state. The system is very easy to use and provides significant time savings.

Gene transfer

siRNA delivery

Small molecule, protein, peptide and probe delivery

Ø Perfectly retained cell function and morphology

Ø No impact on cell viability

Ø Low cell and substrate consumption

Ø Highly efficient, providing significant time savings

Ø No cell processing

Ø Protocols for a wide range of adherent cells

 

 

Transfection with stunning results

Complete system or plug & play module

Superb electroporation has never been easier! Cellaxess ACE is available either as a complete system including pulse generator, or as a stand-alone module which can be combined with almost any pulse generator on the market. Accessing the Cellaxess electroporation platform is easy and highly cost-efficient.

Complete flexibility

Cellaxess ACE can be used with a wide range of cell culture formats - from single

dishes up to 96-well plates. The Cellaxess electrode is exchangeable, which ensures

experimental sterility and prevents the risk of cross contamination. Different

capillaries are available to enable high coverage electroporation in different cell

culture formats.

Ease of use

Cellaxess ACE is extremely easy to use, and offers huge time savings. A single

transfection reaction including preparation takes only a few minutes, an entire

24?well plate can be transfected in less than 15 minutes.

Technical Specifications:

Voltage & power requirements (Cellaxess ACE pulse generator only)

Voltage: 100-240 VAC, 50-60 Hz, CAT II

Power: 200 VA

Computer requirements (Cellaxess ACE pulse generator only)

Microsoft Windows 2000, XP, Vista or 7. Pentium P4 processor, 512 MB of RAM &

10 Mb of available hard-disk space. One available RS232 or USB 1.0 or 2.0 port.

Regulatory compliance

CE certified according to 89/336/EEC for Electromagnetic Compatibility (EC) and

Low-Voltage Directive (73/23/EEC) for product safety.

 

Cellaxess ACE System and

Associated Consumables

Product No: Product Name:

CXA100100 Cellaxess ACE System

CXA100200 Cellaxess ACE Module Kit

CXA200101 Cellaxess ACE capillary electrodes, small, 5-pack1

CXA200102 Cellaxess ACE capillary electrodes, medium, 5-pack2

CXA200103 Cellaxess ACE capillary electrodes, large, 5-pack3

1 Compatible with 384-well plates and lower density cell culture formats

2 Compatible with 96-well plates and lower density cell culture formats

3 Compatible with 48-well plates and lower density cell culture formats

 

 

Cellaxess® ACE

Cellaxess® ACE is a single electrode based electroporation system that can be used for any adherent cell type. Cellaxess® electroporation offers superior transfection quality because of minimal cell processing and low voltages required for electroporation.

Cellaxess® ACE can be used in any cell culture format, and is an excellent match with, high-content and confocal microscopy readouts. Cellaxess® ACE is available either as a complete system including pulse generator, or as a stand-alone module which can be combined with almost any pulse generator on the market.

 

Features

Cellaxess® ACE enables transfection of any adherent cell type. Based on the same concept as Cellaxess® Elektra, the in-situ electroporation at low voltages results in transfections with unsurpassed efficiency and viability. Cellaxess® ACE is ideal for the transfection of challenging cell types, such as primary- and IPS derived neuronal cell types.

Cellaxess® ACE can be used in variety of cell culture formats, ranging from 96-well plates to dishes. It is also an excellent match with high-content and microscopy readouts.

Cellaxess® ACE is available either as a complete system including pulse generator, or as a stand-alone module which can be combined with almost any pulse generator on the market, making it a highly cost efficient solution and an easy way to assess electroporation performance for low volume electroporation.

Cellaxess® ACE has an exchangeable capillary, which ensures sterility in experiments, as well as zero risk of cross contamination. Furthermore, different capillaries can be utilized to accommodate for different cell culture formats.

Benefits优点

Ø Highly efficient transfection of adherent cells such as primary- and IPS cells

高效的转染贴壁的细胞，例如原代和IPS细胞

Ø Perfectly retained cell function and morphology

完美保留了细胞的功能和形态

Ø In-situ cell manipulation directly in cell culture dishes and micro plates

原位细胞操作，直接在细胞培养皿和微板

Ø Little/ no cell processing required

小/没有细胞处理

Ø Low cell consumption

低细胞消耗量

Ø Highly simplified experimental procedures

高度简化实验程序

 

 

Publications

All Cellaxess Dynaflow Company Related

Please click on the product buttons above right, to filter the publications to suit your requirements. Note: Cellaxess®HT was the previous name for Cellaxess® Elektra which has additional functionality for new applications.

Cellaxess

Mitotic Motors Coregulate Microtubule Patterns in Axons and Dendrites Author(s):

Shen Lin 1, Mei Liu 1,2, Olga I. Mozgova 1, Wenqian Yu 1, and Peter W. Baas 1,2

1) Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
2) Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China

Published in: The Journal of Neuroscience, (2012) , 32(40): 14033-14049

High-Throughput Transfection of Differentiated Primary Neurons from Rat Forebrain Author(s):

Shane Marine 1, Jamie Freeman 2, Antonella Riccio 2, Marie-Louise Axenborg 3, Johan Pihl 3, Robin Ketteler 2, and Sara Aspengren 3*

1) Department of Automated Biotechnology, Merck & Co., Inc., North Wales, PA, USA
2) MRC LMCB, University College of London, London, UK
3) *Cellectricon AB, Mölndal, Sweden, Email: sara.aspengren@cellectricon.se

Published in: Journal of Biomolecular Screening | March 8, 2012

Ef?ciency of Cellular Delivery of Antisense Peptide Nucleic Acid by Electroporation Depends on Charge and Electroporation Geometry Author(s):

Mette Joergensen 1, Birgit Agerholm-Larsen 1,2, Peter E. Nielsen 3, and Julie Gehl 1.

1) Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark
2) Department of Neurology, Copenhagen University Hospital Glostrup, Glostrup, Denmark
3) Department of Cellular and Molecular Medicine, Health Science Faculty, University of Copenhagen, Copenhagen, Denmark

Published in: OLIGONUCLEOTIDES, Volume 21, Number 1, 2011

Cellaxess®HT cell-based assay and transfection lab Author(s):

Sara Aspengren*, Michael Tokarz & Johan Pihl

*Cellectricon AB, Flöjelbergsgatan 8C, SE-431 37 Mölndal, Sweden

Published in: NATURE METHODS | JUNE 2011

An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons Author(s):

Catia Andreassi, Carola Zimmermann, Richard Mitter, Salvatore Fusco1, Serena De Vita, Adolfo Saiardi, & Antonella Riccio*

*Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK

Published in: Nature Neuroscience 13, 291 - 301 (2010)

Transfection of chicken cerebellar granule neurons used to study glucocorticoid receptor regulation by nuclear receptor 4A (NR4A Author(s):

Strøm BO, Aden P, Mathisen GH, Lømo J, Davanger S, Paulsen RE.

Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.

Published in: J Neurosci Methods. 2010 Oct 30;193(1):39-46. Epub 2010 Aug 19

FUNCTIONAL DIFFERENCES BETWEEN D1 AND D5 REVEALED BY HIGH RESOLUTION IMAGING ON LIVE NEURONS Author(s):

M. KRUUSMÄGI, S. KUMAR, S. ZELENIN, H. BRISMAR, A. APERIA AND L. SCOTT*

*Department of Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden

Published in: Neuroscience 2009, 164, 463–469

Cellaxess®HT: high-throughput transfection for genomewide RNAi Author(s):

Johan Pihl*, Marie-Louise Johansson, Daniel Granfeldt, Michal Tokarz, Mattias Karlsson & Jon Sinclair

*Cellectricon AB, Flöjelbergsgatan 8C, SE-431 37 Mölndal, Sweden

Published in: Nature Methods - 5, (2008)

Generation of Focused Electric Field Patterns at Dielectric Surfaces Author(s):

Jessica Olofsson(1), Mikael Levin(2), Anette Strömberg(2), Stephen G. Weber(3), Frida Ryttsén(2), and Owe Orwar(1)
1) Department of Chemistry and Bioscience, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Gothenburg, Sweden 3) Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260

Published in: Anal. Chem. 2005, 77, 4667-4672

Fish Eggs Spawn a DNA Delivery Revolution – Caviar inspires a Berkeley bioengineer to take electroporation to the single-cell level Author(s):

Laura lane

Published in: The Scientist 19 (13):34 (4 July 2005)

Electroporation and the Single Cell, New technologies increase the resolution of electroporation Author(s):

Aileen Constans

Published in: The Scientist 18 (8):46 (26 April 2004)

Single-cell electroporation Author(s):

Jessica Olofsson, Kerstin Nolkrantz, Frida Ryttsén, Bradley A. Lambie, Stephen G. Weber, Owe Orwar

Published in: Current Opinion in Biotechnology; 14(1), 29-34 (2003)

Functional Screening of Intracellular Proteins in Single Cells and in Patterned Cell Arrays Using Electroporation Author(s):

Kerstin Nolkrantz, Cecilia Farre, K. Johan Hurtig, Petra Rylander, Owe Orwar

Published in: Anal. Chem. 2002, 74, 4300-4305

Electroporation of Single Cells and Tissues with an Electrolyte-filled Capillary Author(s):

K. Nolkrantz, C. Farre, A. Brederlau, R.I.D. Karlsson, C. Brennan, P.S. Eriksson, S.G. Weber, M. Sandberg, O. Orwar

Published in: Anal. Chem., Sept 15, 73(18), 4469-4477 (2001)

Dynaflow

A method for bidirectional solution exchange – “Liquid bullet” applications of acetylcholine to a7 nicotinic receptors Author(s):

Nikolai Fedorov (1), Lisa Benson (1), John D. Graef (1), Jeremy Hyman (1), Jill Sollenberger (1), Fredrik Pettersson (2), Patrick M. Lippiello (1), Merouane Bencherif (1)

1. Targacept, Inc., Preclinical Research Department, 200 East First Street, Suite #300, Winston Salem, NC 27101, USA
2. Veprox AB, Hängpilsgatan 6, SE-426 77 Västra Frölunda, Sweden

Published in: Journal of Neuroscience Methods, Volume 206, Issue 1, 30 April 2012, Pages 23–33

Alternatively spliced domains interact to regulate BK potassium channel gating Author(s):

Brandon E. Johnson (a), Dominique A. Glausern (a), Elise S. Dan-Glauser (b), D. Brent Halling (c), Richard W. Aldrich (c) and Miriam B. Goodman (a).

a) Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305;
b) Department of Psychology, Stanford University, Stanford, CA 94305
c) Section of Neurobiology, Center for Learning and Memory, University of Texas, Austin, TX 78712

Published in: PNAS December 20, 2011 vol. 108 no. 51 20784-20789

Arrangement of Kv1 alpha subunits dictates sensitivity to tetraethylammonium Author(s):

Al-Sabi A, Shamotienko O, Dhochartaigh SN, Muniyappa N, Le Berre M, Shaban H, Wang J, Sack JT, Dolly JO.

International Centre for Neurotherapeutics, Dublin City University, Dublin 9, Ireland.

Published in: J Gen Physiol. 2010 Sep;136(3):273-82.

Novel Alpha-7 Nicotinic Acetylcholine Receptor Agonists Containing a Urea Moiety: Identification and Characterization of the Potent, Selective, and Orally Efficacious Agonist 1-[6-(4-Fluorophenyl)pyridin-3-yl]-3-(4-piperidin-1-ylbutyl) Urea (SEN34625/WYE-103914) Author(s):

Chiara Ghiron(1) et al;

(1) Siena Biotech SpA, Strada del Petriccio e Belriguardo 35, 53100 Siena, Italy
(2) Chemical Sciences and Neuroscience Discovery Research, Wyeth Research, CN 8000, Princeton, New Jersey 08543-8000

Published in: J. Med. Chem., 2010, 53 (11), pp 4379–4389 DOI: 10.1021/jm901692q

Telithromycin blocks neuromuscular transmission and inhibits nAChR currents in vitro Author(s):

Chang-Ning Liu, Chris J. Somps

Department of Investigative Toxicology, Drug Safety Research & Development, Pfizer Global R&D, Groton, CT 06340, USA

Published in: Toxicol Lett. 2010 May 4;194(3):66-9. Epub 2010 Feb 12.

MEC-2 and MEC-6 in the Caenorhabditis elegans Sensory Mechanotransduction Complex: Auxiliary Subunits that Enable Channel Activity Author(s):

Austin L. Brown(1), Zhiwen Liao(2), and Miriam B. Goodman(1,2)
1) Biophysics Program and 2) Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305

Published in: J. Gen. Physiol., Jun 2008; 131: 605 - 616.

Differential Structure of Atrial and Ventricular KATP Atrial KATP Channels Require SUR1 Author(s):

Thomas P. Flagg(1); Harley T. Kurata(1); Ricard Masia(1); George Caputa(1); Mark A. Magnuson(2); David J. Lefer(3); William A. Coetzee(4); and Colin G. Nichols(1)
1) Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Mo; Department of Molecular Physiology 2) Biophysics, Vanderbilt University School of Medicine, Nashville, Tenn 3) Department of Medicine and Pathology, Albert Einstein College of Medicine, New York; 4) Department of Pediatrics, New York University School of Medicine.

Published in: Circulation Research. 2008 Published online before print October 30, 2008, doi: 10.1161/CIRCRESAHA.108.17818

Role of cAMP sensor Epac as a determinant of K-ATP channel ATP-sensitivity in human pancreatic beta cells and rat INS-1 cells Author(s):

Guoxin Kang, Colin A Leech, Oleg G Chepurny, William A Coetzee, and George G Holz
New York University School of Medicine

Published in: Kang et al. J Physiol.2008; 0: jphysiol.2007.143818v1

Gain-of-Function Mutations in the MEC-4 DEG/ENaC Sensory Mechanotransduction Channel Alter Gating and Drug Blockade Author(s):

Austin L. Brown(1), Silvia M. Fernandez-Illescas(2), Zhiwen Liao2, and Miriam B. Goodman(1,2)
1) Biophysics Program and 2) Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305

Published in: J. Gen. Physiol., Jan 2007; 129: 161 - 173.

Controlling Desensitized States in Ligand-Receptor Interaction Studies with Cyclic Scanning Patch-Clamp Protocols Author(s):

Daniel Granfeldt(1), Jon Sinclair(2), Maria Millingen(1), Cecilia Farre(2), Per Lincoln(1) and Owe Orwar(1)
1) Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Göteborg, Sweden

Published in: Anal. Chem.; 2006; ASAP Article; DOI: 10.1021/ac060812z

A Biohybrid Dynamic Random Access Memory Author(s):

Jon Sinclair(1), Daniel Granfeldt(1), Johan Pihl(2), Maria Millingen(1), Per Lincoln(1), Cecilia Farre(2), Lena Peterson(3), and Owe Orwar(1)
1) Department of Chemistry and Bioscience and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Göteborg, Sweden 3) Department of Signal and Systems, Chalmers University of Technology, SE-412 96 Göteborg, Sweden

Published in: J. Am. Chem. Soc.; 128(15),5109-5113 (doi:10.1021/ja0580993), (2006)

An isoflurane- and alcohol-insensitive mutant GABAA receptor 1 subunit with near normal apparent affinity for GABA: characterization in heterologous systems and production of knock-in mice. Author(s):

Cecilia M. Borghese (1), David F. Werner (2), Norbert Topf (3), Nicole V. Baron (3), L. Ashley Henderson (4), Stephen L. Boehm II (5), Yuri A. Blednov (4), Abdallah Saad (6), Shuiping Dai (6), Robert A. Pearce (6), R. Adron Harris (1), Gregg E. Homanics (2), Neil L. Harrison(3)
1) University of Texas – Austin 2) University of Pittsburgh 3) Weill Medical College of Cornell University 4) University of Texas at Austin 5) State University of New York – Binghamton 6) University of Wisconsin – Madison

Published in: J. Pharmacol. Exp. Ther. 2006: jpet.106.104406v1

Blocking Characteristics of hERG, hNav1.5, and hKvLQT1/ hminK after Administration of the Novel Anti-Arrhythmic Compound AZD7009 Author(s):

Frida Persson, M.Sc.,Leif Carlsson, Ph.D., Göran Duker, Ph.D., and Ingemar Jacobson, Ph.D.
AstraZeneca R&D Mölndal, Mölndal, Sweden

Published in: J Cardiovasc Electrophysiol, 16(3), 329-341,(2005)

Microfluidic device for creating gradients Author(s):

Rajendrani Mukhopadhyay

Published in: Anal. Chem. A-Pages ; 2005; 77(13); 245A-245A.

A chemical waveform synthesizer Author(s):

Jessica Olofsson(1), Helen Bridle(1), Jon Sinclair(1), Daniel Granfeldt(1), Eskil Sahlin(2), and Owe Orwar(1)
1) Department of Chemistry and Bioscience and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden
Edited by Richard N. Zare, Stanford University, Stanford, CA

Published in: PNAS; 102(23), 8097-8102, (doi:10.1073/pnas.0500230102), (2005)

Microfluidic technologies in drug discovery Author(s):

Johan Pihl, Mattias Karlsson, Daniel T. Chiu

Published in: Drug Discovery Today, Vol 10, Number 20, October 2005

Patch clamp electrophysiology steps up a gear Author(s):

Derek J. Trezise, Assay & Compound Profiling, GlaxoSmithKline Research & Development

Published in: European Pharmaceutical review, Issue 2, 2005

Microfluidic Gradient Generating Device for Pharmacological Profiling Author(s):

Johan Pihl(1), Jon Sinclair(1), Eskil Sahlin(2), Mattias Karlsson(3), Fredrik Petterson(3), Jessica Olofsson(1), and Owe Orwar(1)
1) Department of Chemistry and Bioscience, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden 3) Cellectricon AB, Fabriksgatan 7 SE-412 50 Göteborg, Sweden

Published in: Analytical Chemistry; 77(13), 3897-3903, (doi:10.1021/ac050218+), (2005)

Technology Feature: Cell biology, Maximizing return. Ion channels, stem cells and cell signalling are the focus of intense interest in both cell biology and drug discovery. Pete Moore takes a look at what’s on offer for the researcher. Author(s):

Pete Moore

Published in: Nature, Dec 1, 439 (6817), p697-699 (2005). doi:10.1038/438699b

Blocking characteristics of hKv1.5 and hKv4.3/hKChIP2.2 after administration of the novel antiarrhythmic compound AZD7009 Author(s):

Persson, Frida MSc; Carlsson, Leif PhD; Duker, Goran PhD; Ingemar Jacobson PhD
AstraZeneca R&D Mölndal, Mölndal, Sweden

Published in: Journal of Cardiovascular Pharmacology 46(1): 7-17. (2005).

Microfluidics and Patch-Clamp Based Sensors Author(s):

Cecilia Farre, Mattias Karlsson, Jon Sinclair

Published in: Genetic Engineering News, Vol. 24, No 21, December 2004

A Microfluidics Approach to the Problem of Creating Separate Solution Environments Accessible from Macroscopic Volumes Author(s):

Jessica Olofsson(1),Johan Pihl(1),Jon Sinclair(3),Eskil Sahlin(2), Mattias Karlsson(2), and Owe Orwar(1)
1)Department of Chemistry and Bioscience, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden, 2) Cellectricon AB, Fabriksgatan 7 SE-412 50 Göteborg, Sweden, and 3) Department of Chemistry, Gothenburg University, SE-412 96 Göteborg, Sweden

Published in: Analytical Chemistry; 76(17), 4968 - 4976, (doi:10.1021/ac035527j), (2004)

Stabilization of High-Resistance Seals in Patch-Clamp Recordings by Laminar Flow Author(s):

Jon Sinclair, Jessica Olofsson, Johan Pihl, and Owe Orwar
Department of Physical Chemistry and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden

Published in: Analytical Chemistry; 75(23), 6718-6722, (2003), (Technical Note)

Automated Electrophysiology: High Throughput of Art Author(s):

Xiaobo Wang, Min Li

Published in: ASSAY and Drug Development Technologies. Oct 2003, Vol. 1, No. 5: 695-708

A Cell-Based Bar Code Reader for High-Throughput Screening of Ion Channel-Ligand Interactions Author(s):

Jon Sinclair(1),Johan Pihl(1), Jessica Olofsson(1), Mattias Karlsson(1), Kent Jardemark(3), Daniel T. Chiu(2), and Owe Orwar(1)
1) Department of Physical Chemistry, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, University of Washington, Seattle, Washington 98195-1700 3) Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden

Published in: Analytical Chemistry; Dec 15, 74(24), 6133-6138 (2002)

Screening of ion channel receptor agonists using capillary electrophoresis-patch clamp detection with resensitized detector cells Author(s):

Cecilia Farre(1), Andreas Sjöberg(1), Kent Jardemark(1), Ingemar Jacobson(2), and Owe Orwar(1)
Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden, and AstraZeneca AB, SE-431 83 Mölndal, Sweden

Published in: Anal. Chem., Mar 15, 73(6), 1228-33 (2001)

Cellectrion Related

Life Science Technologies Microfluidics—Bringing New Things to Life Science Author(s):

Jeffrey M. Perkel

Published in: AAAS/Science, 6 November 2008

Cellectricon: Insights from inside Author(s):

Ludger Wess

Published in:

Selective introduction of antisense oligonucleotides into single adult CNS progenitor cells using electroporation demonstrates the requirement of STAT3 activation for CNTF-induced gliogenesis Author(s):

Åberg MA, Ryttsen F, Hellgren G, Lindell K, Rosengren LE, MacLennan AJ, Carlsson B, Orwar O, Eriksson PS.

Published in: Mol. Cell. Neurosci., Mar, 17(3), 426-43 (2001)

Chemical transformations in individual ultrasmall biomimetic containers Author(s):

D. T. Chiu, C. F. Wilson, F. Ryttsén, A. Strömberg, C. Farre, A. Karlsson, S. Nordholm, A. Gaggar, B. P. Modi, A. Moscho, R. A. Garza-López, O. Orwar, R. N. Zare

Published in:

Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applicatons Author(s):

M. Karlsson, K. Nolkrantz, M. J. Davidson, A. Strömberg, F. Ryttsén, B. Åkerman, O. Orwar

Published in: Anal. Chem., 72(23), 5857-5862 (2000)

Manipulating the Genetic Identity and Biochemical Surface Properties of Individual Cells with Electric-Field-Induced Fusion Author(s):

A. Strömberg, F. Ryttsén, D. T. Chiu, M. Davidson, P. S. Eriksson, C. F. Wilson, O. Orwar, R. N. Zare

Published in: PNAS, 97(1), 7-11(2000)

Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy Author(s):

Ryttsen F, Farre C, Brennan C, Weber SG, Nolkrantz K, Jardemark K, Chiu DT, Orwar O.

Published in: Biophys. J., Oct; 79(4): 1993-2001 (2000)

Altering the Biochemical State of Individual Cultured Cells and Organelles with Ultramicroelectrodes Author(s):

J. A. Lundqvist, F. Sahlin, M. A. I. Åberg, A. Strömberg, P. S. Eriksson, O. Orwar

Published in: PNAS, 95, 10356-10360 (1998)

 

 

Testimonials

"We have now a new electroporation procedure using the Cellaxess® system for hard-to-transfect neurons. Our obtained result on transient transfection of adherent primary neurons is published in: J Neurosci Methods. 2010"

Prof. Ragnhild E. Paulsen, Oslo University

"Thanks to the unique features of the Cellaxess® technology we are very pleased to have incorporated the technology into our research. Cellaxess® enables electroporation of hard-to-transfect cells and the results we obtained on striatum slice cultures were published in Neuroscience, 2009"

Dr. Lena Scott, Karolinska Institute

"The Cellaxess® technology enabled us to perform high-efficiency transfection of plasmid DNA and siRNAs in primary sympathetic neurons. The results obtained were published in Nature Neuroscience 2010."

Dr. Catia Andreassi, MRC LMCB, University College London