Product Overview
The
EpiQuik™ Global Histone H3-K27 Tri-Methylation Assay Kit is suitable for specifically measuring global histone H3-K27 tri-methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
WHY CHOOSE THE EPIQUIK™ GLOBAL HISTONE H3-K27 TRI-METHYLATION ASSAY KIT?
- Quick and efficient, which can be finished within 5 hours.
- Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
- Strip microwell format makes the assay flexible: manual or high throughput.
- Simple, reliable, and consistent assay conditions.
Principle & Procedure
The EpiQuik™ Global H3-K27 Tri-Methylation Assay Kit is designed for measuring global histone H3-K27 tri-methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The tri-methylated histone H3-K27 can be recognized with high-affinity antibody. The ratio or amount of tri-methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
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Product Components
GT1 (10x Lysis Buffer)
GT2 (Extraction Buffer)
GT3 (10x Wash Buffer)
GT4 (Histone Buffer)
GT5 (Blocking Buffer)
GT6 (Antibody Buffer)
GT7 (Capture Antibody, 100 µg/ml)*
GT8 (Detection Antibody, 400 µg/ml)*
GT9 (Developing Solution)
GT10 (Stop Solution)
Tri-methyl H3-K27 Control (60 µg/ml)*
8-well Assay Strips (with frame)
User Guide
User Guide & MSDS
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[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name. |
