小样信息：
1、货号gta-2，品名GFP-Trap'' coupled to agarose，规格60ul
2、货号gtma-2，品名GFP-Trap''coupled to magnetic agarose beads，规格60ul
3、货号rta-2，品名RFP-Trap'' coupled to agarose，规格60ul
活动申请：
请把您的姓名、导师姓名、科室电话、手机号码、单位名称、收货地址、发票抬头、小样货号、申请原因等信息发到 lab_china@163.com 邮箱，收到邮件后我们会尽快处理，审核通过后我们会尽快送货或者发货。
One step一步法完成IP | Co-IP | ChIP，让您的免疫沉淀更简易，更便捷，更可靠，更专业！
GFP-Trap_A
产品描述： 与agarose beads结合
particle size ~ 80 µm
存储缓冲液：20% EtOH
储存方法：室温运输，收到后4℃储存一年。请勿冰冻。
Nano-Traps®值得信赖的科研工具
Chromotek的Nano-Trap®基于alpaca的单域抗体片段（VHHS）
√ 小型
√ 极端稳定
√ 70℃仍保持稳定，functional in 2M NaCl or 0.5% SDS
√ 高亲和力
√ 解离常数范围在sub-nM内
√ 高特异性
GFP-Trap® 产品特点（高稳定性、高亲和力）
特异性结合绿色荧光蛋白的衍生物，如GFPS65T，YPF或EYFP，不与DsRed衍生蛋白结合。（见FAQs）
√ 多功能且功能强大的GFP融合蛋白生化实验
√ 缩短孵化时间（只需5-30min）
√ 从细胞提取物或细胞器中定量分离融合蛋白及结合（暂时性）因子
√ 超低的非特异性结合影响
√ 无污染的普通抗体的轻链与重链
√ 适用于染色质免疫沉淀（ChIP）
GFP-Trap® 产品应用：
√ 免疫沉淀 IP / Co-IP
√ 质谱分析
√ 酶活测定
√ 生物大分子相互作用/亲和力实验
√ 染色质免疫沉淀（ChIP，Chromatin Immunoprecipitation）
Nano-Traps reliable research reagents
NanoTraps are based on recombinant single domain antibody fragments (VHHS) derived from
alpaca coupled to monovalent matrices (agarose beads'' magnetic particles or multiwell plates).
Our GFPTrap® / RFPTrap® are novel high quality tools for the fast'' reliable and efficient onestep
isolation of fluorescent fusion proteins and their interacting factors.
Features
· Robust and versatile tools for biochemical analyses of fluorescent fusion proteins
· Short incubation times (5 – 30 min)
· Quantitative isolation of fusion proteins and transiently bound factors from various cell extracts
and organelles (from all organisms)
· Low unspecific binding
· No unspecific binding or contamination by heavy and light chains of conventional antibodies
GFP-Trap®
Our GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (agarose beads''
magnetic particles or in 96-multiwell plates) for biochemical analysis of GFP fusion proteins and
their interacting partners.
The GFP-Trap® specifically binds to most common GFP derivates (see FAQs for a complete list).
No binding to red fluorescent proteins derived from DsRed can be detected.
GFP-Trap® features at a glance:
· Robust and versatile tool for biochemical analyses of GFP-fusion proteins
· Short incubation times (5 – 30 min)
· Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
· Low unspecifi c binding
· No contaminating heavy and light chains of conventional antibodies
· Applicable in Chromatin Immunoprecipitation (ChIP)
Use GFP-Trap® for:
· Pulldowns/ Immunoprecipitations
· Co-IP
· Mass spectroscopy
· Enzyme activity measurements
· ChIP analysis
GFP-Trap_A
Description
GFP-Trap® coupled to agarose beads
particle size ~ 80 µm
Storage buffer
20% EtOH
Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one
year. Do not freeze.
Latest references
- Castello'' A. Fischer'' B. Eichelbaum'' K. Horos'' R. Beckmann'' B. Strein'' C. Davey'' N. Humphreys'' D. Preiss'' T. Steinmetz'' L. Krijgsveld'' J Hentze'' M. (2012). Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins. Cell (PubMed)
- Ries'' J. Kaplan'' C. Platonova'' E. Eghidi'' H. Ewers'' H. (2012). A simple'' versatile method for GFP-base super-resolution microscopy via nanobodies. Nat. Methods. 10.1038. (PubMed)
- Pichler'' G. Jack'' A. Wolf'' P. Hake'' S. B. (2012). Versatile Toolbox for High Throughput Biochemical and Functional Studies with Fluorescent Fusion Proteins. PLoS One. 7: e36967. (PubMed)
- Gudesblat'' G. E. Schneider-Pizon'' J. Betti'' C. Mayerhofer'' J. Vanhoutte'' I. van Dongen'' W. Boeren'' S. Zhiponova'' M. de Vries'' S. Jonak'' C. Russinova'' E. (2012). SPEECHLESS integrates brassinosteroid and stomata signalling pathways. Nat Cell Biol. (PubMed)
- Majumdar'' A. Cesario'' W. C. White-Grindley'' E. Jiang'' H. Ren'' F. Khan'' M. R. Li'' L. Choi'' E. M. Kannan'' K. Guo'' F. Unruh'' J. Slaughter'' B. Si'' K. (2012). Critical role of amyloid-like oligomers of Drosophila Orb2 in the persistence of memory. Cell. 148: 515-29. (PubMed)
- Metzger'' T. Gache'' V. Xu'' M. Cadot'' B. Folker'' E. S. Richardson'' B. E. Gomes'' E. R. Baylies'' M. K. (2012). MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. Nature. (PubMed)
- Wild'' P. Farhan'' H. McEwan'' D. G. Wagner'' S. Rogov'' V. V. Brady'' N. R. Richter'' B. Korac'' J. Waidmann'' O. Choudhary'' C. Dotsch'' V. Bumann'' D. Dikic'' I. (2011). Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science. 333: 228-33. (PubMed)
北京乐博生物联系方式：
全国免费咨询热线：4006130504
电话：010-50934026/50934036/52405563
传真：010-58850899
邮箱：info@lab-bio.com
网站：www.lab-bio.com
地址：北京市海淀区清河三街95号同源大厦912室
