来宝网移动站

艾美捷萤火虫荧光素酶测定试剂盒中英文介绍

Firefly Luciferase is widely used as a reporter gene to study gene regulation and function and drug screening. Since there is no endogenous Luciferase activity in mammalian cells or tissues, it is a very sensitive genetic reporter gene. Firefly Luciferase is a 62000 Dalton protein, which is active as a monomer, and does not require subsequent processing of its activity. This enzyme catalyzes the oxidation of ATP dependent D-luciferin to oxidized luciferin, producing light emission. The firefly Luciferase follows the Michaelis Menten kinetics, so the maximum light output can be achieved only when the substrate and cofactor are in large excess. When analyzing under these conditions, the light emitted by the reaction is proportional to the number of Luciferase molecules.

Note: This is a flash type luminescence detection that requires the signal to be measured immediately after adding the working solution to the sample. It requires a single tube luminescent meter or luminescent enzyme reader with a reagent syringe. Biotium also offers the Steady Luc HTS Firefly detection kit (product number 30028L), which is a homogeneous luminescent detection with a signal half-life of 3-5 hours.

Easy to use for consistent results

The firefly Luciferase detection kit is designed to simply and effectively quantify the activity of firefly Luciferase reporter enzyme in cultured cells, with high sensitivity and linearity. This kit is a new improved substitute for the Firefly Luciferase Test Kit (Catalog No. 30003). It adopts Firefly Assay Buffer 2.0, which has better working solution stability compared to the original detection buffer.

Other commercial Luciferase tests require you to dilute all firefly substrates in the test buffer, resulting in a limited shelf life after first use. In Biotium's reagent kit, the substrate is provided as a separate stock solution, which is freshly diluted for each test to achieve excellent shelf life and provide consistent signals over time.

The measurement size is based on the 96 hole format. This kit includes sufficient cell lysis buffer to perform the specified number of measurements within the size range of 96 well to 24 well plates.

萤火虫荧光素酶测定试剂盒2.0是用于转染细胞裂解物中萤火虫荧光素酶活性的闪光型测定。该试剂盒是我们最初的萤火虫荧光素酶测定（目录号30003）的改进版本，具有更好的工作溶液稳定性。150次测定。

艾美捷萤火虫荧光素酶测定试剂盒2.0,150次测定#BTM-30085-1组件：

5X 萤火虫萤光素酶裂解缓冲液

萤火虫萤光素酶检测缓冲液 2.0

D-荧光素

萤火虫荧光素酶测定试剂盒相关特征：

高灵敏度和线性闪光型测定

每次检测都添加新鲜的底物，以确保信号随时间推移保持一致

高性价比

该萤火虫荧光素酶测定试剂盒属性：

化验类型/选项：终点测定，发光（闪光型）

检测方法/读数：光度计（带试剂注射器的单管或微量板读取器）

储存条件：在-60°C或更低温度下储存

来源：https://www.amyjet.com/products/BTM-30085-1.shtml

——————————————————————

艾美捷弗洛拿缓冲的生理盐水( VBS)中英文介绍

含Ca++和Mg++的弗洛拿缓冲的生理盐水（VBS）相关研究：

Cat #

B114-250mL; B115-1L

名称

VBSo (VBSo without Ca++ and Mg++)不含Ca++和Mg++的弗洛拿缓冲的生理盐水

成分

5mM Veronal, 145mM NaCl, 0.025% NaN3, pH 7.3.

应用

当需要不含载体蛋白的缓冲液来稀释蛋白质或作为补体测定的缓冲液时，可使用VBSo。VBSo也可用于制备GVBo、GVB++和GVBE，或者在明胶干扰测定的情况下，它可用于制备含有不同载体蛋白的类似补体缓冲液。

Complement Technology（CompTech）在补体相关试剂领域拥有丰富经验，可提供多种类型的补体研究缓冲液。

明胶弗洛拿缓冲液(Gelatin Veronal Buffer, GVB)和弗洛拿缓冲的生理盐水(Veronal Buffered Saline, VBS)是补体研究的必备缓冲液, 弗洛拿缓冲的生理盐水(VBS)和明胶弗洛拿缓冲液(GVB)相比缺少明胶组分。   

艾美捷弗洛拿缓冲的生理盐水(Veronal Buffered Saline, VBS)解决方案：

编号 B112-250mL; B113-1L

名称 VBS++ (with Ca++ and Mg++)含Ca++和Mg++的弗洛拿缓冲的生理盐水

成分 5mM Veronal, 145mM NaCl, 0.025% NaN3, pH7.3. Containing 0.15mM calcium chloride and 0.5mM magnesium chloride

应用 当需要不含载体蛋白的缓冲液来稀释蛋白质或作为补体测定的缓冲液时，可以使用VBS++。VBS++也可用于制备GVBo, GVB++和GVBE，或者在明胶干扰测定的情况下，它可用于制备包括不同载体蛋白的类似补体缓冲液。

编号 B114-250mL; B115-1L

名称 VBSo (VBSo without Ca++ and Mg++)不含Ca++和Mg++的弗洛拿缓冲的生理盐水

成分 5mM Veronal, 145mM NaCl, 0.025% NaN3, pH 7.3.

应用 当需要不含载体蛋白的缓冲液来稀释蛋白质或作为补体测定的缓冲液时，可使用VBSo。VBSo也可用于制备GVBo、GVB++和GVBE，或者在明胶干扰测定的情况下，它可用于制备含有不同载体蛋白的类似补体缓冲液。

A study on physiological saline (VBS) containing Ca++and Mg++in Flora buffer:

Cat #

B114-250mL;  B115-1L

name

VBSo (VBSo without Ca++and Mg++) Flora buffered saline without Ca++and Mg++

Ingredients

5mM Veronal, 145mM NaCl, 0.025% NaN3, pH 7.3.

Application

When a buffer without carrier protein is needed to dilute the protein or serve as a buffer for complement determination, VBSo can be used. VBSo can also be used to prepare GVBo, GVB++, and GVBE, or in the case of gelatin interference, it can be used to prepare similar complement buffers containing different carrier proteins.

CompTech has extensive experience in the field of complement related reagents and can provide various types of complement research buffers.

Gelatin Verona Buffer (GVB) and Verona Buffered Saline (VBS) are essential buffers for complement research. Compared to Gelatin Verona Buffer (GVB), Verona Buffered Saline (VBS) lacks gelatin components.

The Vernal Buffered Saline (VBS) solution for Emmettefloxacin:

B112-250mL; B113-1L

Name VBS++(with Ca++and Mg++) Flora buffered saline containing Ca++and Mg++

Ingredients: 5mM Veronal, 145mM NaCl, 0.025% NaN3, pH7.3 Containing 0.15mM calcium chloride and 0.5mM magnesium chloride

When a buffer without carrier protein is needed to dilute protein or serve as a buffer for complement determination, VBS++can be used. VBS++can also be used to prepare GVBo, GVB++, and GVBE, or in the case of gelatin interference assay, it can be used to prepare similar complement buffer solutions including different carrier proteins.

B114-250mL; B115-1L

Name VBSo (VBSo without Ca++and Mg++) Flora buffered saline without Ca++and Mg++

Ingredients: 5mM Veronal, 145mM NaCl, 0.025% NaN3, pH 7.3

When a buffer without carrier protein is needed to dilute protein or serve as a buffer for complement determination, VBSo can be used. VBSo can also be used to prepare GVBo, GVB++, and GVBE, or in the case of gelatin interference, it can be used to prepare similar complement buffers containing different carrier proteins.

来源：https://www.amyjet.com/brand/gvb.shtml

——————————————————————

艾美捷SynaptoGreen C4#70020中英文介绍

Amyjet SynaptoGreen C4 # 70020, a widely used green fluorescent dye, is used to track synaptic activity by staining synaptic vesicle at synapses or neuromuscular junctions. When combined with the red fluorescent dye SynaptoRed ™  When used in combination, synapses or neuromuscular junctions can be independently imaged in two colors. The research results of Tsien and Kay's laboratory indicate that when using SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) to reduce background staining, brain slices can be synaptoGreen ™ Staining.

SynaptoGreen C4 attribute:

For live or fixed cells: for live/intact cells

Detection type/option: Real time imaging

Color: Green

CAS number: 149838-22-2

Excitation/emission: 480/598 nm (in film)

Detection of cell localization: membrane/vesicle

Biotium provides a variety of nerve endings staining kits for staining brain slices. Please also refer to AM1-43 (70024), which is compatible with SynaptoGreen ™  Similar, but with amine groups that can immobilize formaldehyde.

SynaptoGreen ™  It can also be used to stain bacteria (Gram positive or Gram negative) and yeast, although it quickly internalizes into the vacuole membrane in yeast. For more information on how Biotium dyes stain various organisms, please refer to its cell staining table.

SynaptoGreen C4已用于标记EM网格上生长的培养神经元，适用于荧光标记、活体光学显微镜和成像研究等。

一种适合于在突触和神经肌肉接头处监测突触活动的荧光神经末梢染料

SynaptoGreen C4注意事项：

保存和操作过程中注意避光

在水溶液中基本无荧光，最大发射波长具pH依赖性。苯乙烯染料的光谱特征在甲醇或氯仿中测定。其在膜环境中的最大激发和最大发射波长都会变短。最大激发波长的差异通常在20nm，发射波长的差异通常是80nm，但依具体的探针有所差异。

艾美捷SynaptoGreen C4#70020 ，一种广泛使用的绿色荧光染料，用于通过对突触或神经肌肉接头处的突触小泡进行染色来跟踪突触活动。当与红色荧光染料 SynaptoRed™ ( 70021 ) 结合使用时，突触或神经肌肉接头可以以两种颜色独立成像。Tsien 和 Kay 实验室的研究结果表明，当使用 SR101（Sulforhodamine 101、80101）或 ADVASEP-7（70029）来减少背景染色时，脑切片可以被SynaptoGreen ™染色。

SynaptoGreen C4属性：

对于活细胞或固定细胞：对于活细胞/完整细胞

检测类型/选项：实时成像

颜色：绿

CAS号：149838-22-2

激发/发射：480/598 nm（在膜中）

探测细胞定位：膜/囊泡

Biotium 提供多种用于染色脑切片的神经末梢染色试剂盒。另请参见 AM1-43 ( 70024 )，它与 SynaptoGreen™ 类似，但具有可固定甲醛的胺基。

SynaptoGreen™ 也可用于对细菌（革兰氏阳性或革兰氏阴性）和酵母进行染色，尽管在酵母中它会迅速内化到液泡膜中。有关Biotium的染料如何染色各种生物体的更多信息，请参阅它的细胞染色表 。

来源：https://www.amyjet.com/products/BTM-70020.shtml

——————————————————————————

艾美捷Calcofluor白色染色剂，含有更高浓度的几丁质

Calcofluor white staining agent can also stain Gram positive bacteria, but cannot stain Gram negative bacteria or mammalian cells. For more information on how our dyes stain various organisms, please refer to Biotium's cell staining table.

Saccharomyces cerevisiae stained with yeast vitality staining kit: Calcofluor white staining agent stained all cell walls blue, ViaVac ™  Color the vacuoles of metabolic active cells red and the cytoplasm of all cells green

Calcofluor white is a staining agent that binds to chitin and cellulose in yeast and fungal cell walls.

Amyjet Calcofluor White Staining Agent # 29067 Features:

Fluorescent blue dye

Binding with cellulose and chitin

Used to stain the cell walls of fungi, algae, and plants

5 mM aqueous solution

C 40 H 44 N 12 O 10 S 2

Calcofluor white dye is a fluorescent blue dye that can bind to cellulose and chitin, which are present in the cell walls of fungi, algae, and plants. In budding yeast, the Calcofluor white stain stains the bud scars more strongly because they contain higher concentrations of chitin. Calcofluor white staining is sometimes used to identify fungal infections in medical samples.

Calcofluor白色是一种与酵母和真菌细胞壁中的几丁质和纤维素结合的染色剂。

Calcofluor白色染色剂是一种荧光蓝色染料，可与纤维素和几丁质结合，后者存在于真菌、藻类和植物的细胞壁中。在出芽酵母中，Calcofluor 白色染色剂对芽痕的染色更强烈，因为它们含有更高浓度的几丁质。Calcofluor 白色染色剂 有时用于识别医学样本中的真菌感染。

艾美捷Calcofluor白色染色剂#29067特征：

荧光蓝色染料

与纤维素和几丁质结合

用于染色真菌、藻类和植物的细胞壁

5 mM 水溶液

C 40 H 44 N 12 O 10 S 2

Calcofluor白色染色剂还可以染色革兰氏阳性菌，但不能染色革兰氏阴性菌或哺乳动物细胞。 有关我们的染料如何对各种生物进行染色的更多信息，请参阅Biotium的细胞染色表。

用酵母活力染色试剂盒染色的酿酒酵母：Calcofluor白色染色剂将所有细胞壁染成蓝色，ViaVac™ 将代谢活跃细胞的液泡染成红色，将所有细胞的细胞质染成绿色

来源：https://www.amyjet.com/products/BTM-29067.shtml

————————————————————————

艾美捷UV/448可固定活性染色试剂盒，活性染料共价标记

How does this work

Live-or-Dye Fixed Viability Stains are amine reactive dyes that are impermeable to cell membranes. Dyes can enter dead cells that have damaged membrane integrity and covalently label free amines on intracellular proteins. Dye labeling is very stable and can immobilize and penetrate cells without losing fluorescence or dye transfer between cells. Live-or-Dye The staining scheme has been optimized to maximize the differentiation of live/dead cells, with minimal staining of live cells to prevent interference with immune staining.

Biotium has multiple Live or Dye options Reactive dyes are available for selection, covering the fluorescence spectrum, and achieving maximum flexibility in designing multi-color flow plates.

Biotium also offers two sampler kits, each containing five different dye colors, for excitation from all popular flow cytometry laser lines. Live-or-Dye The Sampler Kit, Standard (32016) is designed for the most common flow cytometry laser and filter configurations, where dyes can be excited by UV, Violet, Blue, Yellow Green, and Red lasers. Live-or-Dye The Sampler Kit, Spectral (32017) is designed for spectral scanning flow cytometry. It contains dyes that can be excited by ultraviolet, violet, blue, and red lasers, all of which have been demonstrated in Cytek ®  The Aurora spectral cell analyzer was validated and selected due to its ability to fill many gaps in the spectral flow panel.

Live-or-Dye Fixed Viability Stains have dead cell specificity for all cell types, including mammalian cells, bacteria, and yeast.

UV/448可固定活性染色试剂盒允许通过流式细胞术或显微镜来区分活细胞和死细胞。在通过流式细胞术分析细胞表面蛋白表达时，活/死染色是有用的探针，因为它们允许将来自具有可渗透质膜的死细胞的细胞内荧光信号排除在分析之外。

艾美捷UV/448可固定活性染色试剂盒（200次测定）#32002组件：

Live-or-Dye™ 可固定死细胞染料

无水二甲基亚砜

采样器套件组件

5 种不同的 Live-or-Dye™ 可固定死细胞染料

无水二甲基亚砜

UV/448可固定活性染色试剂盒特征：

高度死细胞特异性

用活性染料进行共价标记

承受固定和透化

多种颜色选择

专为光谱流设计的染料选项

方便的采样器套件

UV/448可固定活性染色试剂盒，涵盖荧光光谱，在多色分析中实现非常大的灵活性。

来源：https://www.amyjet.com/products/BTM-32002.shtml

————————————————————————

艾美捷Firefly HTS检测试剂盒，高通量均质分析

Firefly Luciferase is widely used as a reporter gene to study gene regulation and function and drug screening. Due to the lack of endogenous activity in mammalian cells or tissues, it is a highly sensitive genetic reporter gene. However, the light generated by the reaction leads to the formation of suicidal adenosyloxyfluorescein on the surface of the enzyme. It results in a very short half-life of light emission with flashing dynamics. Because of this, flash type luminescence must be detected immediately after the reaction begins, which is incompatible with batch analysis of samples.

Steady Luc from Biotium ™  The Firefly HTS detection kit contains a proprietary mixture of substances that can prevent adenosine oxyfluorescein reactions and generate persistent luminescent signals. It generates a luminescent signal with a half-life of approximately 3 hours for high-throughput microplate detection.

Compared to flash type determination, Steady Luc ™  Isoluminescent Luciferase assay has low luminescence signal. The sensitivity and detection limit of this determination will depend on the expression level of Luciferase in your experimental system and the sensitivity of the photometer.

Biotium的Steady-Luc™HTS检测系统是一种专有的混合物，可通过防止在酶表面形成腺苷酰氧化荧光素来改变酶促反应，从而产生持久的信号（稳定发光）。 它是一种同质的高灵敏度萤火虫荧光素酶报告基因测定试剂盒，具有约3小时的半衰期，用于定量哺乳动物细胞中的萤火虫荧光素酶表达，允许在微孔板中进行批量处理。与闪光型检测相比，发光型荧光素酶检测（如Steady-Luc™）的发光信号较低。 检测的灵敏度和极限将取决于实验系统中的荧光素酶表达水平以及光度计灵敏度。

Biotium还提供Steady-Luc™Firefly HTS检测试剂盒（冻干），冻干检测缓冲液便于室温运输和-20°C储存。

艾美捷Firefly HTS检测试剂盒#BTM-30028-1组件：

D-荧光素

Steady-Luc™ 萤火虫检测缓冲液

Firefly HTS检测试剂盒属性：

化验类型/选项：高通量分析、均质分析、发光（发光型）

检测方法/读数：微孔板读取器（发光）

储存条件：在-60°C或更低温度下储存

Firefly HTS检测试剂盒是一种均相、高灵敏度的萤火虫荧光素酶报告基因检测试剂盒。它是一种发光型检测，信号半衰期约为 3 小时，可在微孔板中进行批处理。

特征：

简单的单步均相测定

发光半衰期约3小时

物超所值：与其他品牌相比节省高达45%

来源：https://www.amyjet.com/products/BTM-30028-1.shtml

————————————————————————

艾美捷海肾荧光素酶测定试剂盒的应用和操作流程

海肾荧光素酶(Renilla luciferase)催化的发光反应，具有很好的浓度线性范围，酶的检测灵敏度达到150次。本试剂盒采用5X 被动裂解缓冲液，能与其他类型的报告基因检测和蛋白含量检测兼容；可与Fire-Lucy Assay Kit共同使用进行一体化的双荧光素酶检测。

艾美捷海肾荧光素酶测定试剂盒2.0,150次测定#BTM-30082-1组件：

5X 被动裂解缓冲液

海肾萤光素酶检测缓冲液 2.0

Aquaphile™ 腔肠素

海肾荧光素酶测定试剂盒相关特征：

敏感、快速；

兼容其它报告基因检测。

海肾荧光素酶测定试剂盒应用范围：

用于海肾荧光素酶检测；

特别适合于高通量筛选技术。

操作基本流程：

1.收取转染细胞，加裂解液裂解。

2.按一定比例混合，配制发光检测液。

3.样品中加入发光检测液，测定酶催化的RLU。

来源：https://www.amyjet.com/products/BTM-30082-1.shtml

————————————————————————————

补体研究bi备缓冲液丨艾美捷多种明胶弗洛拿缓冲液(GVB)

CompTech has extensive experience in the field of complement related reagents and can provide various types of complement research buffers.

Gelatin Verona Buffer (GVB) and Verona Buffered Saline (VBS) are essential buffers for complement research. Compared to Gelatin Verona Buffer (GVB), Verona Buffered Saline (VBS) lacks gelatin components.

Gelatin Verona Buffer (GVB):

B102-250mL; B100-1L

Name GVB++(with Ca++and Mg++) Gelatin Flora Buffer Containing Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3 Containing 0.15mM calcium chloride and 0.5mM magnesium chloride

The application should use GVB++to prepare antibody sensitized sheep red blood cells (EA) and clean them before use for testing; GVB++can be used to dilute all components of complement classical pathway and lectin pathway experiments.

B103-250mL; B101-1L

Name GVBo (GVB without Ca++and Mg++) Gelatin Flora Buffer without Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3

Apply GVBo to prepare rabbit red blood cells (Er) and clean them before use for assay; GVBo can be used to dilute all components of the complement bypass pathway test.

B104-250mL; B105-1L

Name GVBE (GVBo with EDTA) Gelatin Flora Buffer with EDTA

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, 10mM EDTA, pH7.3

The application of GVBE is usually used as a buffer added at the end of the assay to prevent complement activation and dilute the reaction to a level that can be read by spectrophotometry. It is also used to establish a control experiment where complement activation should not occur.

B110-250mL; B111-1L

Name SGVB++(with Ca++and Mg++) Gelatin Flora Buffer Containing Sucrose, Ca++, and Mg++

Ingredients: 170mM Sucrose, 0.1% gelatin, 5mM Vernal, 57mM NaCl, 0.025% NaN3, 0.15mM calcium chloride, and 0.5mM magnesium chloride, pH 7.3

SGVB++can be used to detect C1 in purified C1 complex (product number A098) and normal human serum samples. In these experiments, all components were diluted in SGVB++and EA cells were washed into this buffer. In binding experiments, such as detecting the binding of C5, factor B, or factor H to EsC3b cells (sheep red blood cells with surface bound C3b) or ZymosanC3b cells, it also benefits from being performed in SGVB++. The combination of SGVB++can be enhanced as much as 10 times compared with the use of buffer solutions with physiological ionic strength (such as GVB or PBS).

明胶弗洛拿缓冲液(Gelatin Veronal Buffer, GVB)和弗洛拿缓冲的生理盐水(Veronal Buffered Saline, VBS)是补体研究的必备缓冲液, 弗洛拿缓冲的生理盐水(VBS)和明胶弗洛拿缓冲液(GVB)相比缺少明胶组分。   

艾美捷多种明胶弗洛拿缓冲液(Gelatin Veronal Buffer, GVB)：

编号 B102-250mL; B100-1L

名称 GVB++ (with Ca++ and Mg++)含Ca++和Mg++的明胶弗洛拿缓冲液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3. Containing 0.15mM calcium chloride and 0.5mM magnesium chloride

应用 应使用GVB++制备抗体致敏羊红细胞(EA)，并在用于测定前对其进行清洗；GVB++可用于稀释补体经典途径和凝集素途径试验的所有成分。

编号 B103-250mL; B101-1L

名称 GVBo (GVB without Ca++ and Mg++)不含Ca++和Mg++的明胶弗洛拿缓冲液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3.

应用 GVBo应用于制备兔红细胞(Er)，并在用于测定前对其进行清洗; GVBo可用于稀释补体旁路途径试验的所有组分。

编号 B104-250mL; B105-1L

名称 GVBE (GVBo with EDTA)含EDTA的明胶弗洛拿缓冲液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3, 10mM EDTA, pH7.3.

应用 GVBE通常用作在测定结束时添加的缓冲液，以阻止补体活化并将反应稀释到可分光光度法读取的水平。它也用于建立不应发生补体活化的对照试验。

编号 B110-250mL; B111-1L

名称 SGVB++ (with Ca++ and Mg++)含蔗糖，Ca++和Mg++的明胶弗洛拿缓冲液

成分 170mM Sucrose, 0.1% gelatin, 5mM Veronal, 57mM NaCl, 0.025% NaN3 , 0.15mM calcium chloride, and 0.5mM magnesium chloride, pH7.3.

应用 SGVB++可用于检测纯化的C1复合物(货号A098)和正常人血清样品中的C1。在这些实验中，所有成分在SGVB++中稀释，并将EA细胞冲洗到该缓冲液中。在结合实验中，如检测C5、因子B或因子H与EsC3b细胞(带有表面结合的C3b的绵羊红细胞)或ZymosanC3b细胞的结合，也受益于在SGVB++中进行。SGVB++与使用具有生理离子强度的缓冲液(如GVB或PBS)相比，结合的增强可多达10倍。

来源：https://www.amyjet.com/brand/gvb.shtml