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MED64应用手册：人类干细胞来源的心肌细胞应用

关键词：
人类干细胞来源的心肌细胞 Human stem cell-derived cardiomyocytes (hSC-CMs) 搏动频率 beat frequency; 场电位时程 field potential duration (FPD)
慢性药物作用 chronotropic drug effects; 药物筛选 drug screening

人类干细胞来源的心肌细胞是制药公司和科研机构在研究中应用得比较多的样本。由于人类干细胞来源的心肌细胞具有和原代人类心肌细胞一样的遗传背景，它们的药理学特性应该非常相似，因此结合人类干细胞来源的心肌细胞与MED64多电极胞外记录系统的平台已成为了研究人类遗传性心肌疾病模型和药物筛选的主流。
只需要将hSC-CMs培养在MED记录电极上，就可以从MED64系统中记录和提取出基本的电生理参数，比如搏动频率、以场电位时程等。另外，由于电极的非侵入特性，稳定的记录可以持续非常长的时间，因此慢性药物的效果也能够在MED64系统上进行测量。不仅如此，MED64还可以使用任意电极输出刺激来Pace hSC-CMs，因此可用来研究频率依赖或使用依赖的药物作用。

参考文献：

1.Abnormal calcium handling properties underlie familial hypertrophic cardiomyopathy pathology in patient-specific induced pluripotent stem cells.
Lan F, Lee AS, Liang P, Sanchez-Freire V, Nguyen PK, Wang L, Han L, Yen M, Wang Y, Sun N, Abilez OJ,Hu S, Ebert AD, Navarrete EG, Simmons CS, Wheeler M, Pruitt B, Lewis R, Yamaguchi Y, Ashley EA, Bers
DM, Robbins RC, Longaker MT, Wu JC.
Cell Stem Cell. 12(1):101-13 (2013)

2.Patient-specific induced pluripotent stem cells as a model for familial dilated cardiomyopathy.
Sun N, Yazawa M, Liu J, Han L, Sanchez-Freire V, Abilez OJ, Navarrete EG, Hu S, Wang L, Lee A, Pavlovic A, Lin S, Chen R, Hajjar RJ, Snyder MP, Dolmetsch RE, Butte MJ, Ashley EA, Longaker MT, Robbins RC, Wu JC.
Sci Transl Med. 4:130 (2012)

3.A novel method of selecting human embryonic stem cell-derived cardiomyocyte clusters for assessment of potential to influence QT interval.
Yamazaki K, Hihara T, Taniguchi T, Kohmura N, Yoshinaga T, Ito M, Sawada K.
J Toxicol In Vitro. 26(2):335-342 (2011)

4.Usefulness of field potential as a marker of embryonic stem cell-derived cardiomyocytes, and endpoint analysis of embryonic stem cell test.
Koseki N, Deguchi J, Yamada T, Funabashi H, Seki T.
Toxicol Sci. 35(6):899-909 (2010)

5.Improvement of the embryonic stem cell test endpoint analysis by use of field potential detection.
Koseki N, Deguchi J, Yamada T, Funabashi H, Seki T.J Toxicol Sci. 35(5):619-29 (2010)

6.Progressive maturation in contracting cardiomyocytes derived from human embryonic stem cells: Qualitative effects on electrophysiological responses to drugs.
Otsuji TG, Minami I, Kurose Y, Yamauchi K, Tada M, Nakatsuji N.Stem Cell Res. 4(3):201-13 (2010)